The complex case of Ceratitis cosyra (Diptera, Tephritidae) and relatives. A DNA barcoding perspective

Virgilio Massimiliano, Manrakhan Aruna, Delatte Hélène, Daneel J.H., Mwatawala Maulid W., Meganck Kenny, Barr N., De Meyer Marc. 2017. The complex case of Ceratitis cosyra (Diptera, Tephritidae) and relatives. A DNA barcoding perspective. Journal of Applied Entomology, 141 (10), n.spéc. Biology and management of tephritid fruit flies in Europe, Africa and the Middle East : pp. 788-797.

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Quartile : Q2, Sujet : ENTOMOLOGY

Abstract : The mango fruit fly, Ceratitis (Ceratalaspis) cosyra, is a major agricultural pest affecting mango production in sub-Saharan Africa. Morphological differences between C. cosyra and five closely related Ceratitis (Ceratalaspis) species (C. discussa, C. pallidula, C. quinaria, C. silvestrii, C. striatella) are subtle, so that their identification often requires specialized expertise. A previous study based on nuclear molecular markers (microsatellites) showed cryptic genetic variation within C. cosyra with separate microsatellite genotypic clusters also occurring in sympatry. This study aimed at verifying whether DNA barcoding can (a) separate C. cosyra from morphologically similar Ceratitis (Ceratalaspis) species and (b) resolve the cryptic genetic variation previously observed within C. cosyra. A subset of 62 C. cosyra specimens previously genotyped at 16 microsatellite loci was subjected to DNA barcoding. This data set was integrated with 130 DNA barcodes of C. cosyra, C. discussa, C. quinaria, C. silvestrii and C. striatella. Neighbor Joining, maximum likelihood and Bayesian tree reconstructions confirmed the presence of two main C. cosyra genotypic groups and resolved well-supported groups corresponding to C. discussa, C. pallidula, C. striatella and to C. quinaria/C. silvestrii. The analysis of morphological characters did not show consistent morphological differences between the two groups of C. cosyra and called into question the morphological characters currently implemented in the identification of C. striatella. These results further support the hypothesis of cryptic speciation within the mango fruit fly and suggest that DNA barcoding represents a suitable complementary tool for the problematic diagnosis of C. cosyra and C. striatella.

Mots-clés Agrovoc : Ceratitis, Identification, Espèce, Taxonomie, Biologie moléculaire, ADN, Microsatellite, Technique analytique, Mangifera indica

Mots-clés géographiques Agrovoc : Côte d'Ivoire, Burundi, Éthiopie, Mozambique, Nigéria, Afrique du Sud, Soudan, Sénégal

Mots-clés complémentaires : Ceratitis cosyra

Classification Agris : H10 - Pests of plants
U30 - Research methods

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Agence(s) de financement européenne(s) : European Commission

Programme de financement européen : FP7

Projet(s) de financement européen(s) : Developing African-European joint collaboration for Science and Technology

Auteurs et affiliations

  • Virgilio Massimiliano, Musée royal de l'Afrique centrale (BEL) - auteur correspondant
  • Manrakhan Aruna, Citrus Research International (ZAF)
  • Delatte Hélène, CIRAD-BIOS-UMR PVBMT (REU)
  • Daneel J.H., Citrus Research International (ZAF)
  • Mwatawala Maulid W., SUA (TZA)
  • Meganck Kenny, Royal Museum for Central Africa (BEL)
  • Barr N., Center for Plant Health, Science and Technology Animal and Plant Health Inspection Service (USA)
  • De Meyer Marc, Musée royal de l'Afrique centrale (BEL)

Source : Cirad-Agritrop (

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