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Natural resistance of the diploid Musa Balbisiana Piang Klutuk Wulung (PKW) banana plant to infectious endogenous Banana streak virus sequences is driven by transcriptional gene silencing

Duroy Pierre-Olivier, Laboureau Nathalie, Seguin Jonathan, Rajendran Rajeswaran, Pooggin Mikhail, Iskra Caruana Marie-Line, Chabannes Matthieu. 2017. Natural resistance of the diploid Musa Balbisiana Piang Klutuk Wulung (PKW) banana plant to infectious endogenous Banana streak virus sequences is driven by transcriptional gene silencing. . INRA. Bordeaux : INRA, Résumé, 1 p. EpiAgro 2017. 3, Bordeaux, France, 18 May 2017/19 May 2017.

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Abstract : The genome of banana (Musa sp.) harbours multiple integrations of Banana streak virus (e BSV), whereas this badnavirus does not require integration for the r eplication of its ds DNA genome. Some endogenous BSV sequences (eBSV), only existing in the Musa balbisiana genome, are infectious by releasing a functional viral genome following stresses such as those existing in in vitro culture and interspecific crosse s context. The structure of these eBSV is much longer than a single BSV genome, composed of viral fragments duplicated and more or less extensively rearranged. Wild M. balbisiana diploid genotypes (BB) such as Pisang Klutuk Wulung (PKW) harbour such infectious eBSV belonging to three widespread species of BSV (Goldfinger-BSGFV, Imové – BSIM V and Obino l'Ewai - BSOLV) but are nevertheless resistant to any multiplication of BSV without any visible virus particles. Using deep sequencing of total siRNAs of PKW we underlined the presence of virus-derived small RNA (vsRNA) from eBSOLV, eBSGFV and eBSIMV by blasting sequences against the 3 BSV species genomes. Interestingly, we showed that hot and cold spots of vsRNA generation do not target similar viral sequences from one eBSV species to the other but are directly correlated with the structure of the integration. vsRNA are enriched in 24-nt class which represe nt about 75% of the total 21-24nt siRNA matching eBSV. We also demonstrated that eBSV are highly methylated in the three different sequence contexts (CG, CHH and CHG) throughout the whole sequence of eBSVs with no difference in methylation profile between siRNA producing and non producing areas. Interestingly, methylation patterns of all three eBSV are similar whereas they are located in different genomic context; eBSOLV being in a TE rich area whereas eBSIMV and eBSGFV are in genes rich region. It seems that eBSV are controlled mainly by epigenetic mechanisms similar to those described for transposable elements (TE). All together, our data indicate that eBSVs in PKW genome are likely silenced at the transcriptional level and this is probably responsible for the natural resistance of this genotype to the activation of such infectious eBSV as well as infection by external BSV particles. (Résumé d'auteur)

Classification Agris : H20 - Plant diseases

Auteurs et affiliations

  • Duroy Pierre-Olivier
  • Laboureau Nathalie, CIRAD-BIOS-UMR BGPI (FRA)
  • Seguin Jonathan, University of Basel (CHE)
  • Rajendran Rajeswaran, University of Basel (CHE)
  • Pooggin Mikhail, University of Basel (CHE)
  • Iskra Caruana Marie-Line, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0003-4486-2449
  • Chabannes Matthieu, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-5754-5982

Source : Cirad-Agritrop (https://agritrop.cirad.fr/584408/)

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