Bioassay to Study the Attachment of Xanthomonas albilineans Attachment on sugarcane leaves

Mensi Imène, Daugrois Jean-Heinrich, Rott Philippe. 2017. Bioassay to Study the Attachment of Xanthomonas albilineans Attachment on sugarcane leaves. Bio-Protocol, 7 (2):e2111, 15 p.

Journal article ; Article de revue à comité de lecture Revue en libre accès total
Published version - Anglais
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Abstract : Sugarcane (interspecific hybrids of Saccharum species) is an economically important crop that provides 70% of raw table sugar production worldwide and contributes, in some countries, to bioethanol and electricity production. Leaf scald, caused by the bacterial plant pathogen Xanthomonas albilineans, is one of the major diseases of sugarcane. Dissemination of X. albilineans is mainly ensured by contaminated harvesting tools and infected stalk cuttings. However, some strains of this pathogen are transmitted by aerial means and are able to survive as epiphytes on the sugarcane phyllosphere before entering the leaves and causing disease. H ere we present a protocol to estimate the capacity of attachment of X. albilineans to sugarcane leaves. Tissue -cultured sugarcane plantlets were immersed in a bacterial suspension of X. albilineans and leaf attachment of X. albilineans was determined by two methods: leaf imprinting (semi-quantitative method) and leaf washing/homogenization (quantitative method). These methods are important tools for evaluating pathogenicity of strains/mutants of the sugarcane leaf scald pathogen.

Mots-clés Agrovoc : Canne à sucre, Agent pathogène, Culture de tissu, Xanthomonas albilineans

Classification Agris : H20 - Plant diseases
F60 - Plant physiology and biochemistry
U30 - Research methods

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Mensi Imène, Higher Agronomic Institute of Chott -Mariem (TUN)
  • Daugrois Jean-Heinrich, CIRAD-BIOS-UMR BGPI (FRA)
  • Rott Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-6085-6159 - auteur correspondant

Source : Cirad-Agritrop (

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