Genome-wide identification and characterization of cacao WRKY transcription factors and analysis of their expression in response to witches' broom disease

Silva Monteiro de Almeida Dayanne, Do Amaral Daniel Oliveira Jordão, Del-Bem Luiz-Eduardo, Bronze dos Santos Emily, Santana Silva Raner José, Peres Gramacho Karina, Vincentz Michel, Micheli Fabienne. 2017. Genome-wide identification and characterization of cacao WRKY transcription factors and analysis of their expression in response to witches' broom disease. PloS One, 12 (10):e0187346, 23 p.

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Abstract : Transcriptional regulation, led by transcription factors (TFs) such as those of the WRKY family, is a mechanism used by the organism to enhance or repress gene expression in response to stimuli. Here, we report on the genome-wide analysis of the Theobroma cacao WRKY TF family and also investigate the expression of WRKY genes in cacao infected by the fungus Moniliophthora perniciosa. In the cacao genome, 61 non-redundant WRKY sequences were found and classified in three groups (I to III) according to the WRKY and zinc-finger motif types. The 61 putative WRKY sequences were distributed on the 10 cacao chromosomes and 24 of them came from duplication events. The sequences were phylogenetically organized according to the general WRKY groups. The phylogenetic analysis revealed that subgroups IIa and IIb are sister groups and share a common ancestor, as well as subgroups IId and IIe. The most divergent groups according to the plant origin were IIc and III. According to the phylogenetic analysis, 7 TcWRKY genes were selected and analyzed by RT-qPCR in susceptible and resistant cacao plants infected (or not) with M. perniciosa. Some TcWRKY genes presented interesting responses to M. perniciosa such as Tc01_p014750/Tc06_p013130/AtWRKY28, Tc09_p001530/Tc06_p004420/AtWRKY40, Tc04_p016130/AtWRKY54 and Tc10_p016570/ AtWRKY70. Our results can help to select appropriate candidate genes for further characterization in cacao or in other Theobroma species. (Résumé d'auteur)

Mots-clés Agrovoc : Theobroma cacao, Génome, Expression des gènes, Phylogénie, Moniliophthora, Transcription génique, Mécanisme de défense cellulaire, Résistance génétique, Séquence nucléotidique, PCR, Arabidopsis thaliana

Mots-clés géographiques Agrovoc : Brésil, Sao Paulo, Bahia

Mots-clés complémentaires : Moniliophthora perniciosa, Séquencage

Classification Agris : F30 - Plant genetics and breeding
H20 - Plant diseases

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Silva Monteiro de Almeida Dayanne, UESC (BRA)
  • Do Amaral Daniel Oliveira Jordão, UESC (BRA)
  • Del-Bem Luiz-Eduardo, UESC (BRA)
  • Bronze dos Santos Emily, UESC (BRA)
  • Santana Silva Raner José, UESC (BRA)
  • Peres Gramacho Karina, CEPLAC (BRA)
  • Vincentz Michel, UNICAMP (BRA)
  • Micheli Fabienne, CIRAD-BIOS-UMR AGAP (BRA)

Source : Cirad-Agritrop (

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