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Viral metagenomics approaches for high-resolution screening of multiplexed arthropod and plant viral communities

François Sarah, Filloux Denis, Fernandez Emmanuel, Ogliastro Mylène, Roumagnac Philippe. 2018. Viral metagenomics approaches for high-resolution screening of multiplexed arthropod and plant viral communities. In : Viral metagenomics: methods and protocols. Pantaleo Vitantonio (ed.), Chiumenti Michela (ed.). New York : Humana Press, pp. 77-95. (Methods in Molecular Biology, 1746, 1746) ISBN 978-1-4939-7682-9

Book chapter
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Abstract : Viral metagenomic approaches have become essential for culture-independent and sequence-independent viral detection and characterization. This chapter describes an accurate and efficient approach to (1) concentrate viral particles from arthropods and plants, (2) remove contaminating non-encapsidated nucleic acids, (3) extract and amplify both viral DNA and RNA, and (4) analyze high-throughput sequencing (HTS) data by bioinformatics. Using this approach, up to 96 arthropod or plant samples can be multiplexed in a single HTS library.

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • François Sarah, INRA (FRA)
  • Filloux Denis, CIRAD-BIOS-UMR BGPI (FRA)
  • Fernandez Emmanuel, CIRAD-BIOS-UMR BGPI (FRA)
  • Ogliastro Mylène, INRA (FRA)
  • Roumagnac Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-5002-6039

Autres liens de la publication

Source : Cirad-Agritrop (https://agritrop.cirad.fr/587709/)

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