Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi

Fisher Matthew C., Ghosh Pria, Shelton Jennifer M. G., Bates Kieran A., Brookes Lola, Wierzbicki Claudia, Rosa Gonçalo M., Farrer Rhys A., Aanensen David M., Alvarado-Rybak Mario, Bataille Arnaud, Berger Lee, Böll Susanne, Bosch Jaime, Clare Frances, Courtois Elodie A., Crottini Angelica, Cunningham Andrew A., Doherty-Bone Thomas M., Gebresenbet Fikirte, Gower David J., Höglund Jacob, James Timothy Y., Jenkinson Thomas S., Kosch Tiffany A., Lambertini Carolina, Laurila Anssi, Lin Chun-Fu, Loyau Adeline, Martel An, Meurling Sara, Miaud Claude, Minting Pete, Ndriantsoa Serge, O'Hanlon Simon J., Pasmans Frank, Rakotonanahary Tsanta, Rabemananjara Falitiana C. E., Ribeiro Luisa P., Schmeller Dirk S., Schmidt Benedikt R., Skerratt Lee F., Smith Freya, Soto-Azat Claudio, Tessa Giulia, Toledo Luís Felipe, Valenzuela-Sánchez Andrés, Verster Ruhan, Vörös Judit, Waldman Bruce, Webb Rebecca J., Weldon Ché, Wombwell Emma, Zamudio Kelly R., Longcore Joyce E., Garner Trenton W. J.. 2018. Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi. Scientific Reports, 8:7772, 8 p.

Journal article ; Article de revue à facteur d'impact Revue en libre accès total
Published version - Anglais
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Abstract : Parasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers as part of the BiodivERsA project RACE; here called the RML protocol. In tandem, we developed a lethal procedure for isolating chytrids from tadpole mouthparts. Reviewing a database of use a decade after their inception, we find that these methods have been applied across 5 continents, 23 countries and in 62 amphibian species. Isolation of chytrids by the non-lethal RML protocol occured in 18% of attempts with 207 fungal isolates and three species of chytrid being recovered. Isolation of chytrids from tadpoles occured in 43% of attempts with 334 fungal isolates of one species (Batrachochytrium dendrobatidis) being recovered. Together, these methods have resulted in a significant reduction and refinement of our use of threatened amphibian species and have improved our ability to work with this group of emerging pathogens.

Classification Agris : L73 - Animal diseases
M40 - Aquatic ecology
U30 - Research methods

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Fisher Matthew C., Imperial College London (GBR) - auteur correspondant
  • Ghosh Pria, Imperial College London (GBR)
  • Shelton Jennifer M. G., Imperial College London (GBR)
  • Bates Kieran A., Imperial College London (GBR)
  • Brookes Lola, Institute of Zoology (GBR)
  • Wierzbicki Claudia, Imperial College London (GBR)
  • Rosa Gonçalo M., Institute of Zoology (GBR)
  • Farrer Rhys A., Imperial College London (GBR)
  • Aanensen David M., Imperial College London (GBR)
  • Alvarado-Rybak Mario, Universidad Nacional Andres Bello (CHL)
  • Bataille Arnaud, CIRAD-BIOS-UMR ASTRE (FRA)
  • Berger Lee, James Cook University (AUS)
  • Böll Susanne, Agency for Population Ecology and Nature Conservancy (DEU)
  • Bosch Jaime, Museo Nacional de Ciencias Naturales (ESP)
  • Clare Frances, Imperial College London (GBR)
  • Courtois Elodie A., Université de Guyane (GUF)
  • Crottini Angelica, CIBIO (PRT)
  • Cunningham Andrew A., Zoological Society of London (GBR)
  • Doherty-Bone Thomas M., Royal Zoological Society of Scotland (GBR)
  • Gebresenbet Fikirte, Oklahoma State University (USA)
  • Gower David J., Natural History Museum (GBR)
  • Höglund Jacob, Uppsala University (SWE)
  • James Timothy Y., Endemic Species Research Institute (TWN)
  • Jenkinson Thomas S., University of Michigan (USA)
  • Kosch Tiffany A., Seoul National University (KOR)
  • Lambertini Carolina, UFCG (BRA)
  • Laurila Anssi, Uppsala University (SWE)
  • Lin Chun-Fu, Endemic Species Research Institute (TWN)
  • Loyau Adeline, Helmholtz Centre for Environmental Research (DEU)
  • Martel An, University of Ghent (BEL)
  • Meurling Sara, Uppsala University (SWE)
  • Miaud Claude, Université de Montpellier (FRA)
  • Minting Pete, Amphibian and Reptile Conservation (GBR)
  • Ndriantsoa Serge, Durrell Wildlife Conservation Trust (MDG)
  • O'Hanlon Simon J., Imperial College London (GBR)
  • Pasmans Frank, University of Ghent (BEL)
  • Rakotonanahary Tsanta, Durrell Wildlife Conservation Trust (MDG)
  • Rabemananjara Falitiana C. E., Durrell Wildlife Conservation Trust (MDG)
  • Ribeiro Luisa P., UFCG (BRA)
  • Schmeller Dirk S., Helmholtz Centre for Environmental Research (DEU)
  • Schmidt Benedikt R., University of Zurich (CHE)
  • Skerratt Lee F., James Cook University (AUS)
  • Smith Freya, Institute of Zoology (GBR)
  • Soto-Azat Claudio, Universidad Nacional Andres Bello (CHL)
  • Tessa Giulia, Sardinia Wildlife Conservation (ITA)
  • Toledo Luís Felipe, UNICAMP (BRA)
  • Valenzuela-Sánchez Andrés, Universidad Nacional Andres Bello (CHL)
  • Verster Ruhan, North-West University (ZAF)
  • Vörös Judit, Hungarian Natural History Museum (HUN)
  • Waldman Bruce, Seoul National University (KOR)
  • Webb Rebecca J., James Cook University (AUS)
  • Weldon Ché, North-West University (ZAF)
  • Wombwell Emma, Institute of Zoology (GBR)
  • Zamudio Kelly R., Cornell University (USA)
  • Longcore Joyce E., University of Maine (USA)
  • Garner Trenton W. J., Institute of Zoology (GBR)

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