Vicia faba L. in the Bejaia region of Algeria is nodulated by Rhizobium leguminosarum sv. viciae , Rhizobium laguerreae and two new genospecies

Belhadi Djellali, De Lajudie Philippe, Ramdani Nacer, Le Roux Christine, Boulila Farida, Tisseyre Pierre, Boulil Abdelghani, Benguedouar Amar, Kaci Yahia, Laguerre Gisèle. 2018. Vicia faba L. in the Bejaia region of Algeria is nodulated by Rhizobium leguminosarum sv. viciae , Rhizobium laguerreae and two new genospecies. Systematic and Applied Microbiology, 41 (2) : pp. 122-130.

Journal article ; Article de revue à facteur d'impact
[img] Published version - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.

Télécharger (1MB) | Request a copy


Abstract : Fifty-eight rhizobial strains were isolated from root nodules of Vicia faba cv. Equina and Vicia faba cv. Minor by the host-trapping method in soils collected from eleven sites in Bejaia, Eastern Algeria. Eleven genotypic groups were distinguished based on the combined PCR/RFLP of 16S rRNA, 16S–23S rRNA intergenic spacer and symbiotic (nodC and nodD-F) genes and further confirmed by multilocus sequence analysis (MLSA) of three housekeeping genes (recA, atpD and rpoB), the 16S rRNA gene and the nodulation genes nodC and nodD. Of the 11 genotypes, 5 were dominant and 2 were the most represented. Most of the strains shared high nodD gene sequence similarity with Rhizobium leguminosarum sv. viciae; their nodC sequences were similar to both Rhizobium leguminosarum and Rhizobium laguerreae. Sequence analyses of the 16S–23S rRNA intergenic spacer showed that all the new strains were phylogenetically related to those described from Vicia sativa and V. faba in several African, European, American and Asian countries, with which they form a group related to Rhizobium leguminosarum. Phylogenetic analysis based on MLSA of 16S rRNA, recA, atpD and rpoB genes allowed the affiliations of strain AM11R to Rhizobium leguminosarum sv. viciae and of strains EB1 and ES8 to Rhizobium laguerreae. In addition, two separate clades with <97% similarity may represent two novel genospecies within the genus Rhizobium.

Mots-clés géographiques Agrovoc : Algérie

Mots-clés libres : Rhizobium, Vicia faba, Diversity, Housekeeping genes, Algeria

Classification Agris : F30 - Plant genetics and breeding
P34 - Soil biology

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Belhadi Djellali, Université de Bejaia (DZA) - auteur correspondant
  • De Lajudie Philippe, IRD (FRA)
  • Ramdani Nacer, Université de Bejaia (DZA)
  • Le Roux Christine, CIRAD-BIOS-UMR LSTM (FRA) ORCID: 0000-0003-0668-3589
  • Boulila Farida, Université de Bejaia (DZA)
  • Tisseyre Pierre, IRD (FRA)
  • Boulil Abdelghani, Université de Bejaia (DZA)
  • Benguedouar Amar, Université des Frères Mentouri Constantine 1 (DZA)
  • Kaci Yahia, USTHB (DZA)
  • Laguerre Gisèle, INRA (FRA)

Source : Cirad-Agritrop (

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2021-05-09 ]