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The eggplant AG91-25 recognizes the Type III-secreted effector RipAX2 to trigger resistance to bacterial wilt (Ralstonia solanacearum species complex)

Morel Arry, Guinard Jérémy, Lonjon Fabien, Sujeeun Lakshmi, Barberis Patrick, Genin Stéphane, Vailleau Fabienne, Daunay Marie-Christine, Dintinger Jacques, Poussier Stéphane, Peeters Nemo, Wicker Emmanuel. 2018. The eggplant AG91-25 recognizes the Type III-secreted effector RipAX2 to trigger resistance to bacterial wilt (Ralstonia solanacearum species complex). Molecular Plant Pathology, 19 (11) : pp. 2459-2472.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Url - jeu de données : https://www.ncbi.nlm.nih.gov/nuccore/MF055715 / Url - jeu de données : https://www.ncbi.nlm.nih.gov/nuccore/MF055746

Quartile : Q1, Sujet : PLANT SCIENCES

Abstract : To deploy durable plant resistance, we must understand its underlying molecular mechanisms. Type III effectors (T3Es) and their recognition play a central role in the interaction between bacterial pathogens and crops. We demonstrate that the Ralstonia solanacearum species complex (RSSC) T3E ripAX2 triggers specific resistance in eggplant AG91‐25, which carries the major resistance locus EBWR9. The eggplant accession AG91‐25 is resistant to the wild‐type R. pseudosolanacearum strain GMI1000, whereas a ripAX2 defective mutant of this strain can cause wilt. Notably, the addition of ripAX2 from GMI1000 to PSS4 suppresses wilt development, demonstrating that RipAX2 is an elicitor of AG91‐25 resistance. RipAX2 has been shown previously to induce effector‐triggered immunity (ETI) in the wild relative eggplant Solanum torvum, and its putative zinc (Zn)‐binding motif (HELIH) is critical for ETI. We show that, in our model, the HELIH motif is not necessary for ETI on AG91‐25 eggplant. The ripAX2 gene was present in 68.1% of 91 screened RSSC strains, but in only 31.1% of a 74‐genome collection comprising R. solanacearum and R. syzygii strains. Overall, it is preferentially associated with R. pseudosolanacearum phylotype I. RipAX2GMI1000 appears to be the dominant allele, prevalent in both R. pseudosolanacearum and R. solanacearum, suggesting that the deployment of AG91‐25 resistance could control efficiently bacterial wilt in the Asian, African and American tropics. This study advances the understanding of the interaction between RipAX2 and the resistance genes at the EBWR9 locus, and paves the way for both functional genetics and evolutionary analyses.

Mots-clés libres : Bacterial wilt, Disease resistance, Functional genetics, Plant-pathogenic bacteria, Solanum melongena, Host-pathogen interactions, Plant immunity

Classification Agris : H20 - Plant diseases
F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Agence(s) de financement européenne(s) : European Commission

Auteurs et affiliations

  • Morel Arry, Université de Toulouse (FRA)
  • Guinard Jérémy, CIRAD-BIOS-UMR PVBMT (REU)
  • Lonjon Fabien, Université de Toulouse (FRA)
  • Sujeeun Lakshmi, Université de la Réunion (REU)
  • Barberis Patrick, Université de Toulouse (FRA)
  • Genin Stéphane, INRA (FRA)
  • Vailleau Fabienne, Université de Toulouse (FRA)
  • Daunay Marie-Christine, INRA (FRA)
  • Dintinger Jacques, CIRAD-BIOS-UMR PVBMT (REU)
  • Poussier Stéphane, CIRAD-BIOS-UMR PVBMT (REU)
  • Peeters Nemo, Université de Toulouse (FRA) - auteur correspondant
  • Wicker Emmanuel, CIRAD-BIOS-UMR IPME (FRA) ORCID: 0000-0003-0927-7404 - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/589859/)

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