The multiplicity of cellular infection changes depending on the route of cell infection in a plant virus

Gutierrez Serafin, Pirolles Elodie, Yvon Michel, Baecker Volker, Michalakis Yannis, Blanc Stéphane. 2015. The multiplicity of cellular infection changes depending on the route of cell infection in a plant virus. Journal of Virology, 89 (18) : pp. 9665-9675.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : VIROLOGY

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Abstract : The multiplicity of cellular infection (MOI) is the number of virus genomes of a given virus species that infect individual cells. This parameter chiefly impacts the severity of within-host population bottlenecks as well as the intensity of genetic exchange, competition, and complementation among viral genotypes. Only a few formal estimations of the MOI currently are available, and most theoretical reports have considered this parameter as constant within the infected host. Nevertheless, the colonization of a multicellular host is a complex process during which the MOI may dramatically change in different organs and at different stages of the infection. We have used both qualitative and quantitative approaches to analyze the MOI during the colonization of turnip plants by Turnip mosaic virus. Remarkably, different MOIs were observed at two phases of the systemic infection of a leaf. The MOI was very low in primary infections from virus circulating within the vasculature, generally leading to primary foci founded by a single genome. Each lineage then moved from cell to cell at a very high MOI. Despite this elevated MOI during cell-to-cell progression, coinfection of cells by lineages originating in different primary foci is severely limited by the rapid onset of a mechanism inhibiting secondary infection. Thus, our results unveil an intriguing colonization pattern where individual viral genomes initiate distinct lineages within a leaf. Kin genomes then massively coinfect cells, but coinfection by two distinct lineages is strictly limited.

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Gutierrez Serafin, CIRAD-BIOS-UMR CMAEE (FRA) ORCID: 0000-0002-5277-7239
  • Pirolles Elodie, INRA (FRA)
  • Yvon Michel, INRA (FRA)
  • Baecker Volker, CNRS (FRA)
  • Michalakis Yannis, CNRS (FRA)
  • Blanc Stéphane, INRA (FRA) - auteur correspondant

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