Transcriptome profiles of nod factor-independent symbiosis in the tropical legume Aeschynomene evenia

Gully Djamel, Czernic Pierre, Cruveiller Stéphane, Mahé Frédéric, Longin Cyrille, Vallenet David, François Philippe, Nidelet Sabine, Rialle Stéphanie, Giraud Eric, Arrighi Jean-François, DasGupta Maitrayee, Cartieaux Fabienne. 2018. Transcriptome profiles of nod factor-independent symbiosis in the tropical legume Aeschynomene evenia. Scientific Reports, 8:10934, 12 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
Published version - Anglais
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Abstract : Nod factors (NF) were assumed to be indispensable for the establishment of a rhizobium-legume symbiosis until the discovery that certain Bradyrhizobium strains interacting with certain Aeschynomene species lack the canonical nodABC genes required for their synthesis. So far, the molecular dialogue between Aeschynomene and its symbionts remains an open question. Here we report a time course transcriptional analysis of Aeschynomene evenia in response to inoculation with Bradyrhizobium ORS278. The NF-independent symbiotic process was monitored at five time points between bacterial infection and nodule maturity. The five time points correspond to three specific events, root infection by crack entry, nodule organogenesis, and the establishment of the nitrogen fixing process. During the third stage, about 80 NCR-like genes and eight symbiotic genes known to be involved in signaling, bacterial infection or nodulation regulation were highly expressed. Comparative gene expression analyses at the five time points also enabled the selection of genes with an expression profile that makes them promising markers to monitor early plant responses to bacteria. Such markers could be used in bioassays to identify the nature of the bacterial signal(s). Our data represent valuable resources for investigation of this Nod factor-independent symbiosis.

Classification Agris : F60 - Plant physiology and biochemistry
F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 1 (2014-2018) - Agriculture écologiquement intensive

Auteurs et affiliations

  • Gully Djamel, IRD (FRA)
  • Czernic Pierre, Université de Montpellier (FRA)
  • Cruveiller Stéphane, Centre national de séquençage (FRA)
  • Mahé Frédéric, CIRAD-BIOS-UMR LSTM (FRA) ORCID: 0000-0002-2808-0984
  • Longin Cyrille, Centre national de séquençage (FRA)
  • Vallenet David, Centre national de séquençage (FRA)
  • François Philippe, Université de Montpellier (FRA)
  • Nidelet Sabine, Université de Montpellier (FRA)
  • Rialle Stéphanie, CNRS (FRA)
  • Giraud Eric, LSTM (FRA)
  • Arrighi Jean-François, IRD (FRA)
  • DasGupta Maitrayee, University of Calcutta (IND)
  • Cartieaux Fabienne, IRD (FRA) - auteur correspondant

Source : Cirad-Agritrop (

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