Ricciuti Emeline, Noumbissie Touko Guy Blaise, Chabannes Matthieu, Caruana Marie-Line.
2019. Quantitative PCR cannot be used in diagnosis of Banana streak virus from banana plants with viral integrations. [P25].
In : Livre des résumés des 17 ème Rencontres de virologie végétale. INRA, CIRAD, CNRS, SFP, SFV
Version publiée
- Anglais
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Résumé : Banana streak virus (BSV) is a badnavirus infecting Musa species and inducing Banana streak disease, affecting plant growth and fruit production. BSV exists under two forms: episomal and/or integrated in host genome (referred as “endogenous BSV”, eBSV). Among these viral integrations, four BSV species (BSGFV, BSIMV, BSOLV, and BSMYV) are known to induce eBSV-derived infections under biotic and abiotic stresses.My PhD project aims to follow eBSV-derived infections kinetics in interspecific Musa hybrids (hosting eBSV sequences), over an entire crop cycle. I used qPCR as a quantitative tool to compare BSV infection between different samples, and across time. Because sequence similarity between eBSV and BSV overcomes 99%, I used relative quantification between infected and healthy samples of the same banana genotype hosting eBSV. I refer the healthy, viral particles-free samples as “calibrator”. Viral DNA amplification in the calibrator was supposed to be constant, since eBSV DNA matrix is stable. However, I observed significant variability in eBSV-DNA amplification among a large population of calibrators. This might result from transitory eBSV-DNA expresssion, not encaspidated and therefore escaping serological diagnostics.This result makes this “calibrator” system unreliable for a diagnostic purpose of low infections in banana plants having viral integrations, as standard IC-PCR (immune-capture) and RCA methods have shown better sensibility. Nevertheless, I still use this protocol in my experiments for relative quantification analysis by including a “buffer area” on both sides of the amplification baseline, reflecting viral DNA amplification variations I observed.
Auteurs et affiliations
- Ricciuti Emeline, CIRAD-BIOS-UMR BGPI (FRA)
- Noumbissie Touko Guy Blaise, CARBAP (CMR)
- Chabannes Matthieu, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-5754-5982
- Caruana Marie-Line, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0003-4486-2449
Source : Cirad-Agritrop (https://agritrop.cirad.fr/590981/)
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