A highly specific tool for identification of Xanthomonas vasicola pv. musacearum based on five Xvm-specific coding sequences

Nakato Gloria Valentine, Wicker Emmanuel, Coutinho Teresa A., Mahuku George, Studholme David J.. 2018. A highly specific tool for identification of Xanthomonas vasicola pv. musacearum based on five Xvm-specific coding sequences. Heliyon, 4 (12):e01080, 11 p.

Résumé : Xanthomonas vasicola pv. musacearum (Xvm) is a bacterial pathogen responsible for the economically important Xanthomonas wilt disease on banana and enset crops in Sub-Saharan Africa. Given that the symptoms are similar to those of other diseases, molecular diagnosis is essential to unambiguously identify this pathogen and distinguish it from closely related strains not pathogenic on these hosts. Currently, Xvm identification is based on polymerase chain reaction (PCR) with GspDm primers, targeting the gene encoding general secretory protein D. Experimental results and examination of genomic sequences revealed poor specificity of the GspDm PCR. Here, we present and validate five new Xvm-specific primers amplifying only Xvm strains.

Mots-clés Agrovoc : agent pathogène, organisme nuisible, maladie des plantes, bioinformatique, biologie moléculaire

Mots-clés libres : Bioinformatics, Microbiology, Molecular biology, Plant biology

Classification Agris : H20 - Maladies des plantes
U10 - Informatique, mathématiques et statistiques

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

• Nakato Gloria Valentine, CIRAD-BIOS-UMR IPME (FRA)
• Wicker Emmanuel, CIRAD-BIOS-UMR IPME (FRA) ORCID: 0000-0003-0927-7404 - auteur correspondant
• Coutinho Teresa A., University of Pretoria (ZAF)
• Mahuku George, IITA (UGA)
• Studholme David J., University of Exeter (GBR) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/591122/)

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