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A new prevalent densovirus discovered in Acari. Insight from metagenomics in viral communities associated with two-spotted mite (Tetranychus urticae) populations

François Sarah, Mutuel Doriane, Duncan Alison B., Rodrigues Leonor R., Danzelle Celya, Lefevre Sophie, Santos Inês, Frayssinet Marie, Fernandez Emmanuel, Filloux Denis, Roumagnac Philippe, Froissart Rémy, Ogliastro Mylène. 2019. A new prevalent densovirus discovered in Acari. Insight from metagenomics in viral communities associated with two-spotted mite (Tetranychus urticae) populations. Viruses, 11 (3):233, 24 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
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Quartile : Q2, Sujet : VIROLOGY

Abstract : Viral metagenomics and high throughput sequence mining have revealed unexpected diversity, and the potential presence, of parvoviruses in animals from all phyla. Among arthropods, this diversity highlights the poor knowledge that we have regarding the evolutionary history of densoviruses. The aim of this study was to explore densovirus diversity in a small arthropod pest belonging to Acari, the two-spotted spider mite Tetranychus urticae, while using viral metagenomics based on virus-enrichment. Here, we present the viromes obtained from T. urticae laboratory populations made of contigs that are attributed to nine new potential viral species, including the complete sequence of a novel densovirus. The genome of this densovirus has an ambisens genomic organization and an unusually compact size with particularly small non-structural proteins and a predicted major capsid protein that lacks the typical PLA2 motif that is common to all ambidensoviruses described so far. In addition, we showed that this new densovirus had a wide prevalence across populations of mite species tested and a genomic diversity that likely correlates with the host phylogeny. In particular, we observed a low densovirus genomic diversity between the laboratory and natural populations, which suggests that virus within-species evolution is probably slower than initially thought. Lastly, we showed that this novel densovirus can be inoculated to the host plant following feeding by infected mites, and circulate through the plant vascular system. These findings offer new insights into densovirus prevalence, evolution, and ecology.

Mots-clés Agrovoc : Densovirus, Parvoviridae, Génome, genetic structures [EN], Protéine virale, Ravageur des plantes, Acarien nuisible, Tetranychus urticae, Écologie microbienne

Mots-clés géographiques Agrovoc : Europe

Mots-clés complémentaires : viral genomes (en)

Classification Agris : H10 - Pests of plants

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

  • François Sarah, INRA (FRA)
  • Mutuel Doriane, Université de Montpellier (FRA)
  • Duncan Alison B., Université de Montpellier (FRA)
  • Rodrigues Leonor R., Université de Lisbonne (PRT)
  • Danzelle Celya, Université de Montpellier (FRA)
  • Lefevre Sophie, ISEM (FRA)
  • Santos Inês, Université de Lisbonne (PRT)
  • Frayssinet Marie, INRA (FRA)
  • Fernandez Emmanuel, CIRAD-BIOS-UMR BGPI (FRA)
  • Filloux Denis, CIRAD-BIOS-UMR BGPI (FRA)
  • Roumagnac Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-5002-6039
  • Froissart Rémy, CNRS (FRA)
  • Ogliastro Mylène, INRA (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/591560/)

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