Agritrop
Home

Genome-wide diversity and gene expression profiling of Babesia microti isolates identify polymorphic genes that mediate host-pathogen interactions

Silva Joana C., Cornillot Emmanuel, McCracken Carrie, Usmani-Brown Sahar, Dwivedi Ankit, Ifeonu Olukemi O., Crabtree Jonathan, Gotia Hanzel T., Virji Azan Z., Reynes Christelle, Colinge Jacques, Kumar Vidya, Lawres Lauren, Pazzi Joseph E., Pablo Jozelyn V., Hung Chris, Brancato Jana, Kumari Priti, Orvis Joshua, Tretina Kyle, Chibucos Marcus, Ott Sandy, Sadzewicz Lisa, Sengamalay Naomi, Shetty Amol C., Su Qi, Tallon Luke, Fraser Claire M., Frutos Roger, Molina Douglas M., Krause Peter J., Ben Mamoun Choukri. 2016. Genome-wide diversity and gene expression profiling of Babesia microti isolates identify polymorphic genes that mediate host-pathogen interactions. Scientific Reports, 6:35284, 15 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
[img] Published version - Anglais
License Licence Creative Commons.
Genome Babesia.pdf

Télécharger (2MB)

Quartile : Q1, Sujet : MULTIDISCIPLINARY SCIENCES

Abstract : Babesia microti, a tick-transmitted, intraerythrocytic protozoan parasite circulating mainly among small mammals, is the primary cause of human babesiosis. While most cases are transmitted by Ixodes ticks, the disease may also be transmitted through blood transfusion and perinatally. A comprehensive analysis of genome composition, genetic diversity, and gene expression profiling of seven B. microti isolates revealed that genetic variation in isolates from the Northeast United States is almost exclusively associated with genes encoding the surface proteome and secretome of the parasite. Furthermore, we found that polymorphism is restricted to a small number of genes, which are highly expressed during infection. In order to identify pathogen-encoded factors involved in host-parasite interactions, we screened a proteome array comprised of 174 B. microti proteins, including several predicted members of the parasite secretome. Using this immuno-proteomic approach we identified several novel antigens that trigger strong host immune responses during the onset of infection. The genomic and immunological data presented herein provide the first insights into the determinants of B. microti interaction with its mammalian hosts and their relevance for understanding the selective pressures acting on parasite evolution.

Mots-clés Agrovoc : Babesia microti, Expression des gènes, Variation génétique, Relation hôte pathogène, Ixodes, Sang

Mots-clés géographiques Agrovoc : États-Unis

Classification Agris : L73 - Animal diseases
000 - Other themes

Champ stratégique Cirad : Axe 4 (2014-2018) - Santé des animaux et des plantes

Auteurs et affiliations

  • Silva Joana C., University of Maryland (USA) - auteur correspondant
  • Cornillot Emmanuel, Institut de Biologie Computationnelle (FRA)
  • McCracken Carrie, University of Maryland (USA)
  • Usmani-Brown Sahar, Yale School of Medicine (USA)
  • Dwivedi Ankit, Institut de Biologie Computationnelle (FRA)
  • Ifeonu Olukemi O., University of Maryland (USA)
  • Crabtree Jonathan, University of Maryland (USA)
  • Gotia Hanzel T., University of Maryland (USA)
  • Virji Azan Z., Yale School of Medicine (USA)
  • Reynes Christelle, CNRS (FRA)
  • Colinge Jacques, CRLC (FRA)
  • Kumar Vidya, Yale School of Medicine (USA)
  • Lawres Lauren, Yale School of Medicine (USA)
  • Pazzi Joseph E., Antigen Discovery Inc. (USA)
  • Pablo Jozelyn V., Antigen Discovery Inc. (USA)
  • Hung Chris, Antigen Discovery Inc. (USA)
  • Brancato Jana, Yale School of Medicine (USA)
  • Kumari Priti, University of Maryland (USA)
  • Orvis Joshua, University of Maryland (USA)
  • Tretina Kyle, University of Maryland (USA)
  • Chibucos Marcus, University of Maryland (USA)
  • Ott Sandy, University of Maryland (USA)
  • Sadzewicz Lisa, University of Maryland (USA)
  • Sengamalay Naomi, University of Maryland (USA)
  • Shetty Amol C., University of Maryland (USA)
  • Su Qi, University of Maryland (USA)
  • Tallon Luke, University of Maryland (USA)
  • Fraser Claire M., University of Maryland (USA)
  • Frutos Roger, CIRAD-BIOS-UMR INTERTRYP (FRA)
  • Molina Douglas M., Antigen Discovery Inc. (USA)
  • Krause Peter J., Yale University (USA)
  • Ben Mamoun Choukri, Yale University (USA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/591884/)

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2021-04-08 ]