Wild rodents as potential reservoirs of Trypanosoma in SouthEast Asia: a link towards human infections?

Pumhom Pornpan, Morand Serge, Milocco Cristina, Douangboupha Bounneuang, Jittapalapong Sathaporn, Desquesnes Marc. 2016. Wild rodents as potential reservoirs of Trypanosoma in SouthEast Asia: a link towards human infections?. In : Abstract book of the first International Conference on Non Tsetse Transmitted Animal Trypanosomosis. OIE, ANSES, Institute of Tropical Medicine Antwerp. Paris : OIE, 32., 1 poster International Conference on Non Tsetse Transmitted Animal Trypanosomosis (NTTAT). 1, Paris, France, 14 Décembre 2016/16 Décembre 2016.

Résumé : The present study investigated the molecular prevalence of Trypanosoma lewisi and Trypanosoma evansi in wild rodents from Cambodia, Lao PDR and Thailand. Between 2008 and 2011, rodents were trapped in 10 localities and 584 of them were tested using three sets of primers: TRYP1 (amplifying ITS1 of ribosomal DNA of all trypanosomes), TBR (amplifying satellite genomic DNA of Trypanozoon parasites) and LEW (amplifying ITS1 of ribosomal DNA of Trypanosoma lewisi). Based on the size of the PCR products using TRYP1, 10% were positive for Trypanosoma lewisi and 2.6% positive for Trypanozoon. Results were confirmed by sequencing PCR products and by using more specific primers (LEW and TBR). The specificity of TRYP1 primers however failed as rodent DNA was amplified in some instances. Using LEW, the positive samples for Trypanosoma lewisi were confirmed both by PCR and sequencing. In Thailand, T. lewisi was found in Rattus tanezumi, R exulans and Berylmys; in Lao PDR, in R. tanezumi and R. exulans and in Cambodia in R. tanezumi, R. exulans and R. norvegicus. Using TBR, the positive samples for Trypanozoon were confirmed by sequencing; as T. evansi is the only species of the Trypanozoon sub-genus possibly present in Asian rodents. These results confirmed its presence in rodents from Thailand (R. tanezumi), Lao PDR (R. tanezumi and R. nitidus) and Cambodia (R. tanezumi, Niviventer fulvescens and Maxomys surifer). We tested how habitat structure affects the infection of common murine rodents, inhabiting human-dominated landscapes in South East Asia, by Trypanosoma species. For this, we used geo-reference data of rodents investigated for Trypanosoma infection and land covers developed for seven sites in Thailand, Cambodia and Lao PDR. Infection by T. lewisi was found in rodents living near human settlement and in areas with high cover of built-up habitat. Increased patchiness and high cover of rain-fed agriculture lands were the likely habitat explaining the infection of rodents by T. evansi. These results suggest a likely role of wild rodents as reservoir and possible source of atypical human infection by animal trypanosomes.

Auteurs et affiliations

• Pumhom Pornpan, Kasetsart University (THA)
• Morand Serge, UM2 (FRA) ORCID: 0000-0003-3986-7659
• Milocco Cristina, Kasetsart University (THA)
• Douangboupha Bounneuang, NAFRI (LAO)
• Jittapalapong Sathaporn, Kasetsart University (THA)
• Desquesnes Marc, CIRAD-BIOS-UMR TRYPANOSOMES (THA) ORCID: 0000-0002-7665-2422

Source : Cirad-Agritrop (https://agritrop.cirad.fr/591934/)

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