Agritrop
Home

A duplex quantitative real-time PCR assay for the detection and quantification of Xanthomonas phaseoli pv. dieffenbachiae from diseased and latently infected anthurium tissue

Jouen Emmanuel, Chiroleu Frédéric, Maillot Marie-Véronique, Chabirand Aude, Mérion Sabine, Boyer Claudine, Pruvost Olivier, Robène Isabelle. 2019. A duplex quantitative real-time PCR assay for the detection and quantification of Xanthomonas phaseoli pv. dieffenbachiae from diseased and latently infected anthurium tissue. Journal of Microbiological Methods, 161 : pp. 74-83.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
[img] Published version - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.
MIMET_jouen.pdf

Télécharger (614kB) | Request a copy

Quartile : Q4, Sujet : BIOCHEMICAL RESEARCH METHODS / Quartile : Q4, Sujet : MICROBIOLOGY

Abstract : Anthurium bacterial blight caused by Xanthomonas phaseoli pv. dieffenbachiae (formerly Xanthomonas axonopodis pv. dieffenbachiae) is the major phytosanitary threat in many anthurium growing areas worldwide. Reliable and sensitive diagnostic tools are required for surveillance and certification programs. A duplex real–time quantitative PCR assay was developed for the detection and quantification of X. phaseoli pv. dieffenbachiae from anthurium tissue. This PCR assay targeted a X. phaseoli pv. dieffenbachiae–specific gene encoding an ABC transporter and an internal control encoding for chalcone synthase in Anthurium andreanum. A cycle threshold (Ct), using a receiver-operating characteristic approach (ROC), was implemented to ensure that the declaration of a positive sample was reliable. The duplex real–time assay displayed very high performance with regards to analytical specificity (100% inclusivity, 98.9% exclusivity), analytical sensitivity (LOD95% = 894 bacteria/ml corresponding to 18 bacteria per reaction) and repeatability. We demonstrated the pertinence of this real–time quantitative PCR assay for detecting X. phaseoli pv. dieffenbachiae from diseased leaf tissue (collected from outbreaks on anthurium) and from asymptomatic, latently infected anthurium plants. This assay could be useful for surveillance, as well as for indexing propagative plant material for the presence of X. phaseoli pv. dieffenbachiae.

Mots-clés Agrovoc : Anthurium, Xanthomonas campestris phaseoli, PCR, Maladie bactérienne, Diagnostic, Infection latente

Mots-clés libres : Real time PCR, Xanthomonas phaseoli pv diffenbachiae, Diagnostics, Anthurium, Latent infections

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

  • Jouen Emmanuel, CIRAD-BIOS-UMR PVBMT (REU)
  • Chiroleu Frédéric, CIRAD-BIOS-UMR PVBMT (REU) ORCID: 0000-0002-4874-5357
  • Maillot Marie-Véronique, CIRAD-BIOS-UMR PVBMT (REU)
  • Chabirand Aude, ANSES-LSV (REU)
  • Mérion Sabine, FDGDON (REU)
  • Boyer Claudine, CIRAD-BIOS-UMR PVBMT (REU)
  • Pruvost Olivier, CIRAD-BIOS-UMR PVBMT (REU)
  • Robène Isabelle, CIRAD-BIOS-UMR PVBMT (REU) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/592353/)

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2021-05-02 ]