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High‑throughput sequencing of complete genomes of ipomoviruses associated with an epidemic of cassava brown streak disease in the Comoros Archipelago

Scussel Sarah, Candresse Thierry, Marais Armelle, Claverie Sohini, Hoareau Murielle, Azali Hamza Abdou, Verdin Eric, Tepfer Mark, Filloux Denis, Fernandez Emmanuel, Roumagnac Philippe, Robene Isabelle, Lefeuvre Pierre, Jourda Cyril, Roux-Cuvelier Michel, Lett Jean-Michel. 2019. High‑throughput sequencing of complete genomes of ipomoviruses associated with an epidemic of cassava brown streak disease in the Comoros Archipelago. Archives of Virology, 164 (8) : pp. 2193-2196.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Q3, Sujet : VIROLOGY

Abstract : Using high-throughput sequencing of small interfering RNAs (siRNAs), virion-associated nucleic acid (VANA), and double stranded RNAs (dsRNAs), we have determined the complete genome sequences of Comorian isolates of two ipomoviruses, cassava brown streak virus (CBSV) and a divergent isolate of Ugandan cassava brown streak virus (UCBSV-KM) representing a new strain of this virus. While the large ORF of CBSV shares the highest nucleotide sequence identity (95.9%) with a Tanzanian isolate of CBSV, the large UCBSV-KM ORF shares the highest nucleotide sequence identity (77.5%) with a Malawian isolate of UCBSV. This low value is near the species demarcation threshold for the family Potyviridae (<76%). Phylogenetic analysis confirms that UCBSV-KM represents a new lineage that is genetically distinct from the currently described UCBSV strains.

Mots-clés Agrovoc : Manioc, Potyviridae, Génome, Séquence nucléotidique

Mots-clés géographiques Agrovoc : Comores

Mots-clés complémentaires : Cassava brown streak virus

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agence(s) de financement européenne(s) : European Commission

Auteurs et affiliations

  • Scussel Sarah, CIRAD-BIOS-UMR PVBMT (REU)
  • Candresse Thierry, INRA (FRA)
  • Marais Armelle, INRA (FRA)
  • Claverie Sohini, CIRAD-BIOS-UMR PVBMT (REU)
  • Hoareau Murielle, CIRAD-BIOS-UMR PVBMT (REU)
  • Azali Hamza Abdou, INRAPE (COM)
  • Verdin Eric, INRA (FRA)
  • Tepfer Mark, INRA (FRA)
  • Filloux Denis, CIRAD-BIOS-UMR BGPI (FRA)
  • Fernandez Emmanuel, CIRAD-BIOS-UMR BGPI (FRA)
  • Roumagnac Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-5002-6039
  • Robene Isabelle, CIRAD-BIOS-UMR PVBMT (REU)
  • Lefeuvre Pierre, CIRAD-BIOS-UMR PVBMT (REU)
  • Jourda Cyril, CIRAD-BIOS-UMR PVBMT (REU)
  • Roux-Cuvelier Michel, CIRAD-BIOS-UMR AGAP (GLP)
  • Lett Jean-Michel, CIRAD-BIOS-UMR PVBMT (REU) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/592647/)

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