Comparative proteomics of two citrus varieties in response to infection by the fungus Alternaria alternata

Santos Dória Milena, Silva Guedes Meg, De Andrade Silva Edson Mario, Magalhaes De Oliveira Tahise, Pirovani Carlos Priminho, Kupper Katia Cristina, Bastianel Marinês, Micheli Fabienne. 2019. Comparative proteomics of two citrus varieties in response to infection by the fungus Alternaria alternata. International Journal of Biological Macromolecules, 136 : pp. 410-423.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Quartile : Q1, Sujet : POLYMER SCIENCE / Quartile : Q1, Sujet : CHEMISTRY, APPLIED / Quartile : Q1, Sujet : BIOCHEMISTRY & MOLECULAR BIOLOGY

Abstract : Alternaria brown spot (ABS) is a disease caused by the necrotrophic fungus Alternaria alternata, which induces necrotic lesions on fruits and young leaves due to the production of the host-specific ACT toxin by the fungus. To better understand the citrus–A. alternata interaction and to identify putative resistance proteins, as well as the receptor of the ACT toxin, citrus plants susceptible ('Minneola' mandarin) and resistant ('Clemenules' tangor) to A. alternata, infected or not (control) with the pathogen were analyzed by proteomics. Protein changes were observed between citrus genotypes after infection, and 150 candidate proteins were obtained. A general scheme of the metabolic processes involved in susceptible and resistant citrus–A. alternata interactions was designed. Susceptible plants presented a high level of proteins involved in stress response at the final stages of the infection, whereas resistant plants presented high level of ROS proteins, metabolic proteins, and proteins involved in the immune system process. Proteins like ferredoxin and cyclophilin are specific to the susceptible variety and may be good candidates as fungal effector-interacting proteins. This is the first citrus–A. alternata proteomics analysis, which has allowed a better understanding of the molecular bases of the citrus response to ABS disease.

Mots-clés Agrovoc : Citrus, Génotype, Protéine bactérienne, Résistance aux maladies, Citrus clementina

Mots-clés géographiques Agrovoc : Brésil

Mots-clés complémentaires : Citrus tangerina

Classification Agris : F30 - Plant genetics and breeding
H20 - Plant diseases

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

  • Santos Dória Milena, UESC (BRA)
  • Silva Guedes Meg, UESC (BRA)
  • De Andrade Silva Edson Mario, UESC (BRA)
  • Magalhaes De Oliveira Tahise, UESC (BRA)
  • Pirovani Carlos Priminho, UESC (BRA)
  • Kupper Katia Cristina, IAC (BRA)
  • Bastianel Marinês, UESC (BRA)
  • Micheli Fabienne, CIRAD-BIOS-UMR AGAP (BRA) - auteur correspondant

Source : Cirad-Agritrop (

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