Phylogenetic diversity of Bradyrhizobium strains nodulating Calicotome spinosa in the Northeast of Algeria

Salmi Adouda, Boulila Farida, Bourebaba Yasmina, Le Roux Christine, Belhadi Djellali, De Lajudie Philippe. 2018. Phylogenetic diversity of Bradyrhizobium strains nodulating Calicotome spinosa in the Northeast of Algeria. Systematic and Applied Microbiology, 41 : pp. 452-459.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
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Abstract : Fifty-two slow-growing strains were isolated from root nodules of Calicotome spinosa grown in the Northeast of Algeria and grouped in 24 rep-PCR clusters. One representative strain for each profile was further phylogenetically characterized. The nearly complete 16S rRNA gene sequence indicated that all strains were affiliated to Bradyrhizobium. Multi-Locus Sequence Analysis (MLSA) of the atpD, glnII and recA genes and of the 16S-23S rRNA internal transcribed spacer (ITS) showed that these strains formed four divergent clusters: one close to Bradyrhizobium canariense and Bradyrhizobium lupini and three others separate from all the described species, representing three putative new Bradyrhizobium species. A phylogenetic analysis based on the nodC gene sequence affiliated the strains to either of the two symbiovars, genistearum or retamae.

Mots-clés Agrovoc : Bradyrhizobium, Phylogénie

Mots-clés géographiques Agrovoc : Algérie

Mots-clés libres : Bradyrhizobium, Calicotome spinosa, MLSA, Symbiosis, Root nodulesa

Classification Agris : P34 - Soil biology
F30 - Plant genetics and breeding

Champ stratégique Cirad : Axe 6 (2014-2018) - Sociétés, natures et territoires

Auteurs et affiliations

  • Salmi Adouda, Université de Bejaia (DZA)
  • Boulila Farida, Université de Bejaia (DZA) - auteur correspondant
  • Bourebaba Yasmina, Université de Bejaia (DZA)
  • Le Roux Christine, CIRAD-BIOS-UMR LSTM (FRA) ORCID: 0000-0003-0668-3589
  • Belhadi Djellali, Université de Bejaia (DZA)
  • De Lajudie Philippe, IRD (FRA)

Source : Cirad-Agritrop (

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