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Development of a duplex-PCR for differential diagnosis of Xanthomonas phaseoli pv. manihotis and Xanthomonas cassavae in cassava (Manihot esculenta)

Flores Carolina, Zarate Carlos, Triplett Lindsay, Maillot-Lebon Véronique, Moufid Yassine, Kanté Moussa, Bragard Claude, Verdier Valérie, Gagnevin Lionel, Szurek Boris, Robène Isabelle. 2019. Development of a duplex-PCR for differential diagnosis of Xanthomonas phaseoli pv. manihotis and Xanthomonas cassavae in cassava (Manihot esculenta). Physiological and Molecular Plant Pathology, 105:n.spéc. Crop Pathology in Africa : pp. 34-46.

Journal article ; Article de revue à facteur d'impact
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Quartile : Q2, Sujet : PLANT SCIENCES

Abstract : Cassava Bacterial Blight and Cassava Bacterial Necrosis are two bacterial diseases affecting cassava, respectively caused by Xanthomonas phaseoli pv. manihotis (Xpm) and Xanthomonas cassavae (Xc). Since both pathogens may be present on leaves and cause similar symptoms, we developed a new molecular diagnostic tool to detect and distinguish between Xpm and Xc. Based on genome sequences from the target species as well as from non-target species, in silico analysis was performed to select candidate primers through a novel strategy targeting specificity. Experimental validation enabled to establish a duplex-PCR diagnostic tool that will be useful for future surveillance programs and better disease control.

Mots-clés Agrovoc : Manihot esculenta, Xanthomonas campestris phaseoli, PCR, Maladie bactérienne, Épidémiologie

Mots-clés géographiques Agrovoc : Afrique

Mots-clés complémentaires : Xanthomonas cassavae, Xanthomonas phaseoli

Mots-clés libres : Cassava, Diagnostic PCR, Xanthomonas phaseoli pv. manihotis, Xanthomonas cassavae

Classification Agris : H20 - Plant diseases
U30 - Research methods

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Agence(s) de financement européenne(s) : European Commission

Auteurs et affiliations

  • Flores Carolina, IRD (FRA)
  • Zarate Carlos, IRD (FRA)
  • Triplett Lindsay, Colorado State University (USA)
  • Maillot-Lebon Véronique
  • Moufid Yassine, Université de Montpellier (FRA)
  • Kanté Moussa, Université de Montpellier (FRA)
  • Bragard Claude, UCL (BEL)
  • Verdier Valérie, IRD (FRA)
  • Gagnevin Lionel, CIRAD-BIOS-UMR IPME (FRA)
  • Szurek Boris, IRD (FRA)
  • Robène Isabelle, CIRAD-BIOS-UMR PVBMT (REU) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/593261/)

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