Diversity of dromedary camel coronavirus HKU23 in African camels revealed multiple recombination events among closely related betacoronaviruses of the subgenus embecovirus

So Ray T.Y., Chu Daniel K.W., Miguel Eve, Perera Ranawaka A.P.M., Oladipo Jamiu O., Fassi-Fihri Ouafaa, Aylet Gelagay, Ko Ronald L. W., Zhou Ziqi, Cheng Mo-Sheung, Kuranga Sulyman A., Roger François, Chevalier Véronique, Webby Richard J., Woo Patrick C. Y., Poon Leo L.M., Peiris Malik. 2019. Diversity of dromedary camel coronavirus HKU23 in African camels revealed multiple recombination events among closely related betacoronaviruses of the subgenus embecovirus. Journal of Virology, 93 (23):e01236-19, 18 p.

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Quartile : Q1, Sujet : VIROLOGY

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Abstract : Genetic recombination has frequently been observed in coronaviruses. Here, we sequenced multiple complete genomes of dromedary camel coronavirus HKU23 (DcCoV-HKU23) from Nigeria, Morocco, and Ethiopia and identified several genomic positions indicative of cross-species virus recombination events among other betacoronaviruses of the subgenus Embecovirus (clade A beta-CoVs). Recombinant fragments of a rabbit coronavirus (RbCoV-HKU14) were identified at the hemagglutinin esterase gene position. Homolog fragments of a rodent CoV were also observed at 8.9-kDa open reading frame 4a at the 3′ end of the spike gene. The patterns of recombination differed geographically across the African region, highlighting a mosaic structure of DcCoV-HKU23 genomes circulating in dromedaries. Our results highlighted active recombination of coronaviruses circulating in dromedaries and are also relevant to the emergence and evolution of other betacoronaviruses, including Middle East respiratory syndrome coronavirus (MERS-CoV). IMPORTANCE Genetic recombination is often demonstrated in coronaviruses and can result in host range expansion or alteration in tissue tropism. Here, we showed interspecies events of recombination of an endemic dromedary camel coronavirus, HKU23, with other clade A betacoronaviruses. Our results supported the possibility that the zoonotic pathogen MERS-CoV, which also cocirculates in the same camel species, may have undergone similar recombination events facilitating its emergence or may do so in its future evolution.

Mots-clés Agrovoc : Dromadaire, Coronavirinae, Transmission des maladies, génomique, genomic features [EN]

Mots-clés géographiques Agrovoc : Nigéria, Maroc, Éthiopie, Afrique

Mots-clés libres : Camel, MERS-CoV, Coronavirus, Dromedary

Classification Agris : L73 - Animal diseases
L10 - Animal genetics and breeding

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

  • So Ray T.Y., University of Hong Kong (CHN)
  • Chu Daniel K.W., University of Hong Kong (CHN)
  • Miguel Eve, IRD (FRA)
  • Perera Ranawaka A.P.M., University of Hong Kong (CHN)
  • Oladipo Jamiu O., University of Hong Kong (CHN)
  • Fassi-Fihri Ouafaa, IAV Hassan II (MAR)
  • Aylet Gelagay, Pan African Veterinary Vaccine Centre (ETH)
  • Ko Ronald L. W., University of Hong Kong (CHN)
  • Zhou Ziqi, University of Hong Kong (CHN)
  • Cheng Mo-Sheung, University of Hong Kong (CHN)
  • Kuranga Sulyman A., University of Ilorin (NGA)
  • Roger François, CIRAD-BIOS-UMR ASTRE (REU) ORCID: 0000-0002-1573-6833
  • Chevalier Véronique, CIRAD-BIOS-UMR ASTRE (KHM)
  • Webby Richard J., St Jude Children's Research Hospital (USA)
  • Woo Patrick C. Y., University of Hong Kong (CHN)
  • Poon Leo L.M., University of Hong Kong (CHN)
  • Peiris Malik, University of Hong Kong (CHN) - auteur correspondant

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