Next-generation sequencing on insectivorous bat guano : An accurate tool to identify arthropod viruses of potential agricultural concern

Bourgarel Mathieu, Noel Valérie, Pfukenyi Davies Mubika, Michaux Johan, André Adrien, Becquer Thierry, Cerqueira Frédérique, Barrachina Célia, Boué Vanina, Talignani Loïc, Matope Gift, Missé Dorothée, Morand Serge, Liégeois Florian. 2019. Next-generation sequencing on insectivorous bat guano : An accurate tool to identify arthropod viruses of potential agricultural concern. Viruses, 11:1102, 14 p.

https://doi.org/10.3390/v11121102

Article de revue ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total

Prévisualisation

Version publiée - Anglais
Sous licence

.
Mathieu et al. - 2019 - Next-Generation Sequencing on Insectivorous Bat Guano An Accurate Tool to Identify Arthropod Viruses of Potenti.pdf
Télécharger (4MB) | Prévisualisation

Quartile : Q2, Sujet : VIROLOGY

Résumé : Viruses belonging to the Dicistroviridae family have attracted a great deal of attention from scientists owing to their negative impact on agricultural economics, as well as their recent identification as potential aetiological agents of febrile illness in human patients. On the other hand, some Dicistroviruses are also studied for their potential biopesticide properties. To date, Dicistrovirus characterized in African mainland remain scarce. By using High-Throughput Sequencing technology on insectivorous bat faeces (Hipposideros Caffer) sampled in a cave used by humans to collect bat guano (bat manure) as fertilizer in Zimbabwe, we characterized the full-length sequences of three Dicistrovirus belonging to the Cripavirus and Aparavirus genus: Big Sioux River Virus-Like (BSRV-Like), Acute Bee Paralysis Virus (ABPV), and Aphid Lethal Paralysis Virus (ALPV). Phylogenetic analyses of ORF-1 and ORF-2 genes showed a complex evolutionary history between BSRV and close viruses, as well as for the Aparavirus genus. Herewith, we provide the first evidence of the presence of Dicistrovirus in Zimbabwe and highlight the need to further document the impact of such viruses on crops, as well as in beekeeping activities in Zimbabwe which represent a crucial source of income for Zimbabwean people.

Mots-clés Agrovoc : Chiroptera, guano, fertilisation, transmission des maladies, vecteur de maladie, engrais organique

Mots-clés géographiques Agrovoc : Zimbabwe

Mots-clés libres : Dicistrovirus, Bats, Faeces, Phylogeny, Zimbabwe

Classification Agris : L20 - Écologie animale
L73 - Maladies des animaux
S50 - Santé humaine

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations