Comparative transcriptome profiling of resistant and susceptible sugarcane cultivars in response to infection by Xanthomonas albilineans

Ntambo Mbuya Sylvain, Meng Jian-Yu, Rott Philippe, Henry Robert, Zhang Hui-Li, Gao San-Ji. 2019. Comparative transcriptome profiling of resistant and susceptible sugarcane cultivars in response to infection by Xanthomonas albilineans. International Journal of Molecular Sciences, 20 (24):6138, 20 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
Published version - Anglais
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Abstract : Sugarcane (Saccharum spp. hybrids) is a major source of sugar and renewable bioenergy crop worldwide and suffers serious yield losses due to many pathogen infections. Leaf scald caused by Xanthomonas albilineans is a major bacterial disease of sugarcane in most sugarcane-planting countries. The molecular mechanisms of resistance to leaf scald in this plant are, however, still unclear. We performed a comparative transcriptome analysis between resistant (LCP 85-384) and susceptible (ROC20) sugarcane cultivars infected by X. albilineans using the RNA-seq platform. 24 cDNA libraries were generated with RNA isolated at four time points (0, 24, 48, and 72 h post inoculation) from the two cultivars with three biological replicates. A total of 105,783 differentially expressed genes (DEGs) were identified in both cultivars and the most upregulated and downregulated DEGs were annotated for the processes of the metabolic and single-organism categories, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the 7612 DEGs showed that plant–pathogen interaction, spliceosome, glutathione metabolism, protein processing in endoplasmic reticulum, and plant hormone signal transduction contributed to sugarcane's response to X. albilineans infection. Subsequently, relative expression levels of ten DEGs determined by quantitative reverse transcription-PCR (qRT-PCR), in addition to RNA-Seq data, indicated that different plant hormone (auxin and ethylene) signal transduction pathways play essential roles in sugarcane infected by X. albilineans. In conclusion, our results provide, for the first time, valuable information regarding the transcriptome changes in sugarcane in response to infection by X. albilineans, which contribute to the understanding of the molecular mechanisms underlying the interactions between sugarcane and this pathogen and provide important clues for further characterization of leaf scald resistance in sugarcane.

Mots-clés Agrovoc : Saccharum, Canne à sucre, Xanthomonas albilineans, Expression des gènes, Résistance aux maladies, Relation hôte pathogène

Mots-clés complémentaires : Échaudure des feuilles

Mots-clés libres : Leaf scald, Saccharum spp., Xanthomonas albilineans, Transcriptome analysis, Disease resistance

Classification Agris : H20 - Plant diseases
F30 - Plant genetics and breeding

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

  • Ntambo Mbuya Sylvain, Fujian Agriculture and Forestry University (CHN)
  • Meng Jian-Yu, Fujian Agriculture and Forestry University (CHN)
  • Rott Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-6085-6159
  • Henry Robert, University of Queensland (AUS)
  • Zhang Hui-Li, Fujian Agriculture and Forestry University (CHN)
  • Gao San-Ji, Fujian Agriculture and Forestry University (CHN) - auteur correspondant

Source : Cirad-Agritrop (

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