Experimental study of soft tick vector competence for African Swine Fever in Europe

Pereira De Oliveira Rémi, Hutet Evelyne, Paboeuf Frédéric, Duhayon Maxime, Boinas Fernando, Pérez de León Adalberto A., Filatov Sehrii, Vial Laurence, Le Potier Marie-Frédérique. 2019. Experimental study of soft tick vector competence for African Swine Fever in Europe. In : 13th EPIZONE Annual Meeting "Breaking Walls" - Abstract Book. Mettenleiter Thomas C. (ed.), Beer Martin (ed.), Conraths Franz J. (ed.), Blome Sandra (ed.), Bussmann Bianca M. (ed.). FLI, German Federal Research Institute for Animal Health. Berlin : Friedrich-Loeffler-Institut, Résumé, p. 66. EPIZONE Annual Meeting. 13, Berlin, Allemagne, 26 August 2019/28 August 2019.

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Abstract : African swine fever (ASF) is a lethal viral disease that has spread to Europe and Asia. Infected tick bite is one of the known routes of ASF virus (ASFV) transmission. Some species in the Ornithodoros genus are competent vector for ASFV. However, their role in transmission and maintenance of ASFV currently circulating in Europe remains to be determined. Three Ornithodoros species were selected for transmissions trials: O. moubata (known vector in Africa), O. erraticus (reported vector in Portugal) and O. verrucosus (suspected vector in Ukraine). Four ASFV strains were selected (Liv13/33, OurT88/1, Georgia2007/1 and Ukr12/Zapo) and matched to tick species, which yielded five pairs for vector competence testing: O. moubata-Liv13/33 (OmL), O. moubata-Georgia2007/1 (OmG), O. erraticus-OurT88/1 (OeO), O. erraticus-Georgia2007/1 (OeG) and O. verrucosus-Zapo (OvZ). Tick infection was attempted by feeding on viraemic pigs then re-feeding two to eight months later on healthy pigs to test for virus transmission ability. Only O. moubata was able to transmit ASFV. For other pairs, ticks were crushed and the homogenate of supernatant was injected in naïve pigs. All inoculated pigs developed clinical signs and viremia within a week, confirming ASFV was infectious in these tick-virus pairs. For the first time, the vectorial competence of O. erraticus and O. verrucosus for European strains was tested under experimental conditions and showed the ability of these both ticks to keep the virus infectious after feeding on viraemic pig. These experiments reinforce the importance of studying underlying mechanisms of soft tick vector competence for ASFV.

Auteurs et affiliations

  • Pereira De Oliveira Rémi, CIRAD-BIOS-UMR ASTRE (FRA) ORCID: 0000-0002-3005-1277
  • Hutet Evelyne, ANSES (FRA)
  • Paboeuf Frédéric, ANSES (FRA)
  • Duhayon Maxime, CIRAD-BIOS-UMR ASTRE (FRA)
  • Boinas Fernando, Universidade Técnica de Lisboa (PRT)
  • Pérez de León Adalberto A., Knipling-Bushland US Livestock Insects Research Laboratory (USA)
  • Filatov Sehrii, National Scientific Center Institute of Experimental and Clinical Veterinary Medicine (UKR)
  • Vial Laurence, CIRAD-BIOS-UMR ASTRE (FRA)
  • Le Potier Marie-Frédérique, ANSES (FRA)

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