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A novel divergent geminivirus identified in asymptomatic new world cactaceae plants

Fontenele Rafaela S, Salywon Andrew M., Majure Lucas C., Cobb Ilaria N., Bhaskara Amulya, Avalos-Calleros Jesús, Argüello-Astorga Gerardo, Schmidlin Kara, Khalifeh Anthony, Smith Kendal, Schreck Joshua, Lund Michael, Köhler Matias, Wojciechowski Martin, Hodgson Wendy C., Puente-Martinez Raul, Van Doorslaer Koenraad, Kumari Safaa G., Vernière Christian, Filloux Denis, Roumagnac Philippe, Lefeuvre Pierre, Ribeiro Simone G., Kraberger Simona, Martin Darren Patrick, Varsani Arvind. 2020. A novel divergent geminivirus identified in asymptomatic new world cactaceae plants. Viruses, 12 (4):398, 26 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
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Abstract : Cactaceae comprise a diverse and iconic group of flowering plants which are almost exclusively indigenous to the New World. The wide variety of growth forms found amongst the cacti have led to the trafficking of many species throughout the world as ornamentals. Despite the evolution and physiological properties of these plants having been extensively studied, little research has focused on cactus-associated viral communities. While only single-stranded RNA viruses had ever been reported in cacti, here we report the discovery of cactus-infecting single-stranded DNA viruses. These viruses all apparently belong to a single divergent species of the family Geminiviridae and have been tentatively named Opuntia virus 1 (OpV1). A total of 79 apparently complete OpV1 genomes were recovered from 31 different cactus plants (belonging to 20 different cactus species from both the Cactoideae and Opuntioideae clades) and from nine cactus-feeding cochineal insects (Dactylopius sp.) sampled in the USA and Mexico. These 79 OpV1 genomes all share > 78.4% nucleotide identity with one another and < 64.9% identity with previously characterized geminiviruses. Collectively, the OpV1 genomes display evidence of frequent recombination, with some genomes displaying up to five recombinant regions. In one case, recombinant regions span ~40% of the genome. We demonstrate that an infectious clone of an OpV1 genome can replicate in Nicotiana benthamiana and Opuntia microdasys. In addition to expanding the inventory of viruses that are known to infect cacti, the OpV1 group is so distantly related to other known geminiviruses that it likely represents a new geminivirus genus. It remains to be determined whether, like its cactus hosts, its geographical distribution spans the globe.

Mots-clés Agrovoc : Géminivirus, ssDNA viruses [EN], Dactylopius

Mots-clés géographiques Agrovoc : États-Unis, Mexique

Mots-clés complémentaires : Cactoideae, Opuntioideae

Mots-clés libres : Geminivirus, Cactoideae, Opuntioideae, SsDNA virus, Cochineal insects

Classification Agris : H20 - Plant diseases

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

  • Fontenele Rafaela S, Arizona State University (USA)
  • Salywon Andrew M., Desert Botanical Garden (USA)
  • Majure Lucas C., University of Florida (USA)
  • Cobb Ilaria N., Arizona State University (USA)
  • Bhaskara Amulya, Center for Research in Engineering, Science and Technology (USA)
  • Avalos-Calleros Jesús, Instituto Potosino de Investigacion Cientifica y Tecnologica (MEX)
  • Argüello-Astorga Gerardo, Instituto Potosino de Investigacion Cientifica y Tecnologica (MEX)
  • Schmidlin Kara, Arizona State University (USA)
  • Khalifeh Anthony, Arizona State University (USA)
  • Smith Kendal, Arizona State University (USA)
  • Schreck Joshua, Arizona State University (USA)
  • Lund Michael, Arizona State University (USA)
  • Köhler Matias, UFRGS (BRA)
  • Wojciechowski Martin, Arizona State University (USA)
  • Hodgson Wendy C., Desert Botanical Garden (USA)
  • Puente-Martinez Raul, Desert Botanical Garden (USA)
  • Van Doorslaer Koenraad, Arizona State University (USA)
  • Kumari Safaa G., ICARDA (SYR)
  • Vernière Christian, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0002-2312-2073
  • Filloux Denis, CIRAD-BIOS-UMR BGPI (FRA)
  • Roumagnac Philippe, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0001-5002-6039
  • Lefeuvre Pierre, CIRAD-BIOS-UMR PVBMT (REU)
  • Ribeiro Simone G., EMBRAPA (BRA)
  • Kraberger Simona, Arizona State University (USA)
  • Martin Darren Patrick, Institute of Infectious Diseases and Molecular Medicine (ZAF)
  • Varsani Arvind, Arizona State University (USA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/595493/)

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