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Identification and fine mapping of Pi69(t), a new gene conferring broad-spectrum resistance against Magnaporthe oryzae from Oryza glaberrima steud

Dong Liying, Liu Shuguang, Sandar Kyaing May, Xu Peng, Tharreau Didier, Deng Wei, Li Xundong, Bi Yunqing, Zeng Li, Li Jing, Zhou Jiawu, Tao Dayun, Yang Qinzhong. 2020. Identification and fine mapping of Pi69(t), a new gene conferring broad-spectrum resistance against Magnaporthe oryzae from Oryza glaberrima steud. Frontiers in Plant Science, 11:1190, 8 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact Revue en libre accès total
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Abstract : The discovery and deployment of new broad-spectrum resistance (R) genes from cultivated rice and its wild relatives is a strategy to broaden the genetic basis of modern rice cultivars to combat rice blast disease. Oryza glaberrima possessing many valuable traits for tolerance to biotic and abiotic stresses, is an elite gene pool for improvement of Asian cultivated rice. An introgression line IL106 derived from O. glaberrima (Acc. IRGC100137) confers complete resistance to Magnaporthe oryzae in blast nursery. Genetic analysis using 2185 BC6F2 progenies derived from a cross between IL106 and the recurrent parent Dianjingyou 1 showed that IL106 harbors a single dominant resistance gene against M. oryzae strain 09BSH-10-5A. This gene was preliminarily mapped on the long arm of chromosome 6 of rice in a region of ca. 0.9 cM delimited by two SSR markers (RM20650 and RM20701). In order to finely map this gene, 17,100 additional progenies were further analyzed. As a result, this gene was further narrowed down to a region flanked by two molecular markers STS69-15 and STS69-7, and co-segregated with 3 molecular markers, RM20676, STS69-21 and STS69-22 on the long arm of chromosome 6. Based on reference genome sequences, this R gene was mapped in silico in 76.1-Kb and 67.7-Kb physical intervals, and containing 4 and 3 NBS-LRR candidate genes in O. sativa cultivar Nipponbare and O. glaberrima cultivar CG14, respectively. Because no blast resistance gene was finely mapped in this physical interval before, this R gene was considered as not described yet and designated as Pi69(t), which is the first identified and finely mapped blast R gene from O. glaberrima, as far as we know. Evaluation of IL106 with 151 blast strains collected from 6 countries in Asia showed that 148 strains are avirulent on IL106, suggesting that Pi69(t) is a broad-spectrum blast R gene, and a promising resistant resource for improvement of Asian cultivated rice.

Mots-clés Agrovoc : Oryza glaberrima, Magnaporthe, Pyricularia oryzae, Gène, Résistance aux maladies, Introgression, Expérimentation in vitro

Mots-clés complémentaires : Magnaporthe oryzae, Gène de résistance

Mots-clés libres : Oryza glaberrima, Introgression line, Magnaporthe oryzae, Pyricularia oryzae, Resistance gene, Fine mapping

Classification Agris : F30 - Plant genetics and breeding
H20 - Plant diseases

Champ stratégique Cirad : CTS 1 (2019-) - Biodiversité

Auteurs et affiliations

  • Dong Liying, Yunnan Academy of Agricultural Sciences (CHN)
  • Liu Shuguang, Yunnan Academy of Agricultural Sciences (CHN)
  • Sandar Kyaing May, Yunnan Academy of Agricultural Sciences (CHN)
  • Xu Peng, Yunnan Academy of Agricultural Sciences (CHN)
  • Tharreau Didier, CIRAD-BIOS-UMR BGPI (FRA) ORCID: 0000-0003-3961-6120
  • Deng Wei, Yunnan Academy of Agricultural Sciences (CHN)
  • Li Xundong, Yunnan Academy of Agricultural Sciences (CHN)
  • Bi Yunqing, Yunnan Academy of Agricultural Sciences (CHN)
  • Zeng Li, Yunnan Academy of Agricultural Sciences (CHN)
  • Li Jing, Yunnan Academy of Agricultural Sciences (CHN)
  • Zhou Jiawu, Yunnan Academy of Agricultural Sciences (CHN)
  • Tao Dayun, Yunnan Academy of Agricultural Sciences (CHN) - auteur correspondant
  • Yang Qinzhong, Yunnan Academy of Agricultural Sciences (CHN) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/596316/)

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