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Homeobox transcription factor OsZHD2 promotes root meristem activity in rice by inducing ethylene biosynthesis

Yoon Jinmi, Cho Lae-Hyeon, Yang Wenzhu, Pasriga Richa, Wu Yunfei, Hong Woo-Jong, Bureau Charlotte, Wi Soo Jin, Zhang Tao, Wang Rongchen, Zhang Dabing, Jung Ki-Hong, Park Ky Young, Perin Christophe, Zhao Yunde, An Gynheung. 2020. Homeobox transcription factor OsZHD2 promotes root meristem activity in rice by inducing ethylene biosynthesis. Journal of Experimental Botany, 71 (18) : pp. 5348-5364.

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Abstract : Root meristem activity is the most critical process influencing root development. Although several factors that regulate meristem activity have been identified in rice, studies on the enhancement of meristem activity in roots are limited. We identified a T-DNA activation tagging line of a zinc-finger homeobox gene, OsZHD2, which has longer seminal and lateral roots due to increased meristem activity. The phenotypes were confirmed in transgenic plants overexpressing OsZHD2. In addition, the overexpressing plants showed enhanced grain yield under low nutrient and paddy field conditions. OsZHD2 was preferentially expressed in the shoot apical meristem and root tips. Transcriptome analyses and quantitative real-time PCR experiments on roots from the activation tagging line and the wild type showed that genes for ethylene biosynthesis were up-regulated in the activation line. Ethylene levels were higher in the activation lines compared with the wild type. ChIP assay results suggested that OsZHD2 induces ethylene biosynthesis by controlling ACS5 directly. Treatment with ACC (1-aminocyclopropane-1-carboxylic acid), an ethylene precursor, induced the expression of the DR5 reporter at the root tip and stele, whereas treatment with an ethylene biosynthesis inhibitor, AVG (aminoethoxyvinylglycine), decreased that expression in both the wild type and the OsZHD2 overexpression line. These observations suggest that OsZHD2 enhances root meristem activity by influencing ethylene biosynthesis and, in turn, auxin.

Mots-clés Agrovoc : Homéobox, phytogénétique, Biosynthèse, Éthylène, Oryza sativa, Facteur de transcription, Amélioration des plantes, Méristème apical, Système racinaire, Rendement des cultures

Mots-clés complémentaires : Croissance des plantes, transcriptome

Mots-clés libres : Ethylene biosynthesis, Grain yield, Homeobox transcription factor, Low-nutrient, Rice, Root meristem

Classification Agris : F30 - Plant genetics and breeding
F62 - Plant physiology - Growth and development

Champ stratégique Cirad : CTS 2 (2019-) - Transitions agroécologiques

Auteurs et affiliations

  • Yoon Jinmi, Kyung Hee University (KOR)
  • Cho Lae-Hyeon, Kyung Hee University (KOR)
  • Yang Wenzhu, CAAS (CHN)
  • Pasriga Richa, Kyung Hee University (KOR)
  • Wu Yunfei, Kyung Hee University (KOR)
  • Hong Woo-Jong, Kyung Hee University (KOR)
  • Bureau Charlotte
  • Wi Soo Jin, Sunchon National University (KOR)
  • Zhang Tao, Huazhong Agricultural University (CHN)
  • Wang Rongchen, Huazhong Agricultural University (CHN)
  • Zhang Dabing, Shanghai Jiaotong University (CHN)
  • Jung Ki-Hong, Kyung Hee University (KOR)
  • Park Ky Young, Sunchon National University (KOR)
  • Perin Christophe, CIRAD-BIOS-UMR AGAP (FRA) ORCID: 0000-0002-2469-310X
  • Zhao Yunde, UC (USA)
  • An Gynheung, Kyung Hee University (KOR) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/597989/)

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