Agritrop
Home

Quantitative monitoring of galactolipid hydrolysis by pancreatic lipase-related protein 2 using thin layer chromatography and thymol-sulfuric acid derivatization

Sahaka Moulay, Amara Sawsan, Lecomte Jérôme, Rodier Jean-David, Lafont Dominique, Villeneuve Pierre, Gontero Brigitte, Carrière Frédéric. 2021. Quantitative monitoring of galactolipid hydrolysis by pancreatic lipase-related protein 2 using thin layer chromatography and thymol-sulfuric acid derivatization. Journal of Chromatography. B, 1173:122674, 9 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
[img] Version Online first - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.
Sahaka et al J. Chromatography B 2021.pdf

Télécharger (3MB) | Request a copy

Abstract : Galactolipids are the most abundant lipids on earth where they are mainly found in photosynthetic membranes of plant, algae, and cyanobacteria. Pancreatic lipase-related protein 2 (PLRP2) is an enzyme with galactolipase activity allowing mammals, especially herbivores, to digest this important source of fatty acids. We present a method for the quantitative analysis of galactolipids and galactosylated products resulting from their digestion by guinea pig PLRP2 (GPLRP2), using thin-layer-chromatography (TLC), thymol-sulfuric acid as derivatization reagent and scanning densitometry for detection. Thymol-sulfuric acid reagent has been used for the colorimetric detection of carbohydrates. It is shown here that the derivatization of galactosyl group from galactolipids by this reagent is not affected by the bound acyl glycerol, acyl chains length and number of galactose residues in the polar head. This allowed quantifying simultaneously the initial substrate and all galactosylated products generated upon the hydrolysis of monogalactosyl di-octanoylglycerol (C8-MGDG) by GPLRP2 using a single calibration with C8-MGDG as reference standard. The reaction products, monogalactosyl monooctanoyl glycerol (C8-MGMG) and monogalactosyl glycerol (MGG), were identified and quantified, MGG being recovered from the aqueous phase and analyzed by a separate TLC analysis. This method is therefore suitable to quantify the products resulting from the release of both fatty acids present in MGDG and thereby shows that PLRP2 can contribute to the complete digestion of galactolipids and further intestinal absorption of their fatty acids.

Mots-clés Agrovoc : Chromatographie en couche mince, Analyse quantitative, Galactose, Acide gras, Glycolipide, Réactif chimique, Digestion, Lipolyse

Mots-clés complémentaires : galactolipides

Mots-clés libres : Lipids, Thin layer chromatography, Galactolipids

Classification Agris : L51 - Animal physiology - Nutrition
L50 - Animal physiology and biochemistry

Champ stratégique Cirad : CTS 7 (2019-) - Hors champs stratégiques

Auteurs et affiliations

  • Sahaka Moulay, Université d'Aix-Marseille (FRA)
  • Amara Sawsan, Lipolytech, Zone Luminy Biotech (FRA)
  • Lecomte Jérôme, CIRAD-PERSYST-UMR IATE (FRA)
  • Rodier Jean-David, Gattefossé (FRA)
  • Lafont Dominique, UCLB (FRA)
  • Villeneuve Pierre, CIRAD-PERSYST-UMR IATE (FRA) ORCID: 0000-0003-1685-1494
  • Gontero Brigitte, Université d'Aix-Marseille (FRA)
  • Carrière Frédéric, CNRS (FRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/598007/)

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2021-04-19 ]