Agritrop
Home

Identifying potential candidate Culicoides spp. for the study of interactions with Candidatus Cardinium hertigii

Pilgrim Jack, Siozios S., Baylis Matthew, Venter Gert J., Garros Claire, Hurst Gregory D.D.. 2021. Identifying potential candidate Culicoides spp. for the study of interactions with Candidatus Cardinium hertigii. Medical and Veterinary Entomology, 6 p.

Journal article ; Article de recherche ; Article de revue à facteur d'impact
[img] Version Online first - Anglais
Access restricted to CIRAD agents
Use under authorization by the author or CIRAD.
Pilgrim et al MVE 2021.pdf

Télécharger (550kB) | Request a copy

Url - jeu de données : http://doi.org/10.6084/m9.figshare.13187069 / Url - jeu de données : http://doi.org/10.6084/m9.figshare.13142684 / Url - jeu de données : http://doi.org/10.6084/m9.figshare.13130306

Liste HCERES des revues (en SHS) : oui

Thème(s) HCERES des revues (en SHS) : Psychologie-éthologie-ergonomie

Abstract : Culicoides biting midges (Diptera: Ceratopogonidae) are vectors responsible for the transmission of several viruses of veterinary importance. Previous screens of Culicoides have described the presence of the endosymbiont Candidatus Cardinium hertigii (Bacteroidetes). However, any impacts of this microbe on vectorial capacity, akin to those conferred by Wolbachia in mosquitoes, are yet to be uncovered and await a suitable system to study Cardinium‐midge interactions. To identify potential candidate species to investigate these interactions, accurate knowledge of the distribution of the endosymbiont within Culicoides populations is needed. We used conventional and nested PCR assays to screen Cardinium infection in 337 individuals of 25 Culicoides species from both Palearctic and Afrotropical regions. Infections were observed in several vector species including C. imicola and the Pulicaris complex (C. pulicaris, C. bysta, C. newsteadi and C. punctatus) with varying prevalence. Phylogenetic analysis based on the Gyrase B gene grouped all new isolates within 'group C' of the genus, a clade that has to date been exclusively described in Culicoides. Through a comparison of our results with previous screens, we suggest C. imicola and C. sonorensis represent good candidates for onward study of Cardinium‐midge interactions.

Mots-clés Agrovoc : Culicoides, Rickettsia, Vecteur de maladie, Phylogénie

Mots-clés complémentaires : Candidatus Cardinium hertigii, Culicoides imicola, Culicoides sonorensis

Mots-clés libres : Cardinium, Culicoides, Rickettsia, Symbiosis

Classification Agris : L72 - Pests of animals

Champ stratégique Cirad : CTS 4 (2019-) - Santé des plantes, des animaux et des écosystèmes

Auteurs et affiliations

  • Pilgrim Jack, University of Liverpool (GBR)
  • Siozios S., University of Liverpool (GBR)
  • Baylis Matthew, University of Liverpool (GBR)
  • Venter Gert J., OVI (ZAF)
  • Garros Claire, CIRAD-BIOS-UMR ASTRE (FRA) ORCID: 0000-0003-4378-5031
  • Hurst Gregory D.D., University of Liverpool (GBR) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/598216/)

View Item (staff only) View Item (staff only)

[ Page générée et mise en cache le 2021-06-09 ]