Experimental validation under controlled conditions of real time PCR to quantify arbuscular mycorrhizal colonization in root

Arruda Bruna, Rodrigues Yasmin Florentino, Herrera Wilfrand Ferney Bejarano, Robin Alexandre, Cotta Simone Raposo, Andreote Fernando Dini. 2022. Experimental validation under controlled conditions of real time PCR to quantify arbuscular mycorrhizal colonization in root. Journal of Microbiological Methods, 192:106382, 4 p.

Quartile : Q3, Sujet : BIOCHEMICAL RESEARCH METHODS / Quartile : Q4, Sujet : MICROBIOLOGY

Résumé : Mycorrhizal colonization of roots is traditionally evaluated by empirical methods, such as root microscopy. We compared this method with data from using a real time PCR technique, and determined the correlation between methods, indicating particularities of a promising system for a quick and accurate molecular diagnostic of arbuscular mycorrhization.

Mots-clés Agrovoc : microscopie, PCR, Crotalaria juncea, Mycorhizé

Mots-clés libres : Brachiaria, Crotalaria, Glomeromycota, Microscopy (TEM), QPCR

Classification Agris : F40 - Écologie végétale

Champ stratégique Cirad : CTS 1 (2019-) - Biodiversité

Auteurs et affiliations

• Arruda Bruna, USP (BRA)
• Rodrigues Yasmin Florentino, USP (BRA)
• Herrera Wilfrand Ferney Bejarano, AGROSAVIA (COL)
• Robin Alexandre, CIRAD-BIOS-UMR LSTM (FRA)
• Cotta Simone Raposo, USP (BRA)
• Andreote Fernando Dini, USP (BRA) - auteur correspondant

Source : Cirad-Agritrop (https://agritrop.cirad.fr/601582/)

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