Molecular and biochemical diversity among isolates of Radopholus spp. from different areas of the World

Fallas G.A., Hahn M.L., Fargette Mireille, Burrows P.R., Sarah Jean-Louis. 1996. Molecular and biochemical diversity among isolates of Radopholus spp. from different areas of the World. Journal of Nematology, 28 (4) : 422-430.

Autre titre : Diversité moléculaire et biochimique de Radopholus spp isolé de différentes régions du monde

Résumé : Eleven isolates of #Radopholus similis# from various banana-growing areas around the World and one isolate of #R. bridgei# from turmeric in Indonesia were compared using DNA and isoenzyme analysis. The polymerase chain reaction (PCR) was used to amplify a fragment of ribosomal DNA (rDNA), comprising the two internal transcribed spacers (ITS) and the 5.85 gene. Restriction fragment length polymorphisms (RFLPS) in this rDNA fragment were used to compare the 10 isolates. The analysis of this rDNA region revealed little variation among the isolates tested. However, data also were obtained by random amplified polymorphic DNA (RAPD) analysis of total DNA, and a hierarchical cluster analysis of these data arranged the #R. similis# isolates into two clusters. The first cluster consisted of isolates from Nigeria, Cameroon, Queensland, and Costa Rica; the second was comprised of isolates from Guinea, Guadeloupe, the Ivory Coast, Uganda, and Sri Lanka. The solate of #R. bridgei# from turmeric in Indonesia appeared to be more divergent. This grouping was consistent with that obtained when phosphate glucose isomerase (PGI) isoenzyme patterns were used o compare the #R. similis# isolates. The results from both RAPD analysis and PGI isoenzyme studies indicate that two gene pools might exist within the #R. similis# isolates studied. No correlation could be detected between the genomic diversity as determined by RAPD analysis and either geographic distribution of the isolates or differences in their pathogenicity. The results support the hypothesis that #R. similis isolates# have been spread with banana-planting material.

Mots-clés Agrovoc : Radopholus, Radopholus similis, PCR, marqueur génétique, RFLP, RAPD, variation génétique

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Fallas G.A.
• Hahn M.L.
• Fargette Mireille
• Burrows P.R.
• Sarah Jean-Louis

Source : Cirad - Agritrop (https://agritrop.cirad.fr/389671/)

Métriques

[ Page générée et mise en cache le 2024-12-12 ]