Comparative genetic diversity studies of Theobroma cacao L. using RFLP and RAPD markers

N'Goran Jeanne A.K., Laurent V., Risterucci Ange-Marie, Lanaud Claire. 1994. Comparative genetic diversity studies of Theobroma cacao L. using RFLP and RAPD markers. Heredity, 73 : 589-597.

Autre titre : Etudes comparatives de la diversité génétique de Theobroma cacao L. par marqueurs RFLP et RAPD

Résumé : The genetic diversity of 106 genotypes of #Theobroma cacao# L. was assessed using RFLP and RAPD methods. Thirty-one cDNA probes and 19 primers generated, respectively, 87 and 49 polymorphic bands that were submitted to factorial and cluster analyses. Both RFLP and RAPD bands separated the individuals into three distinctive groups. Some genotypes did not correspond to the currently accepted classification and are considered to have been originally misclassified. The nature of RAPD bands was investigated by Southern analysis. There were as many highly repeated sequences as single copy sequences but the two types of sequences did not structure the variability in the same way. Similar results were obtained with RFLPs and RAPDs with regard to the genetic structure of #Theobioma cacao# L. Therefore, RAPDs appear to be as efficient as RFLPs for classifying cocoa genotypes.

Mots-clés Agrovoc : Theobroma cacao, variation génétique, RFLP, RAPD, technique analytique, méthode statistique

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• N'Goran Jeanne A.K.
• Laurent V.
• Risterucci Ange-Marie
• Lanaud Claire ORCID: 0000-0001-6411-7310

Source : Cirad - Agritrop (https://agritrop.cirad.fr/389738/)

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