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Further studies on RAPD of phloem restricted plant trypanosomatids : comparison between several processings of raw data

Hebrard Eugénie, Dollet Michel. 1997. Further studies on RAPD of phloem restricted plant trypanosomatids : comparison between several processings of raw data. In : International workshop on molecular epidemiology and evolutionary genetics of pathogenic microorganisms. 2nd Session = [Atelier international sur l'épidémiologie moléculaire et le développement génétique de microorganismes pathogènes]. Anon. s.l. : s.n., 1 p. International workshop on molecular epidemiology and evolutionary genetics of pathogenic microorganisms. 2, Montpellier, France, 26 Avril 1997/28 Avril 1997.

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Autre titre : Avancement des études avec RAPD sur les plantes aux phloèmes limités par les trypanosomes : comparaison entre plusieurs classements des données

Résumé : Discovered in 1909, plants trypanosomatids (Protozoa Kinetoplastida) have been immediately classified in an arbitrary genus "#Phytomonas#" to distinguish them from flagellate parasites of mammals. This taxonomic classification is problematic because it puts together organisms which have many biological, biochemical and molecular differences. Plant trypanosomatids scientists now agree to separate "#Phytomonas#" in three main groups including phloem restricted trypanosomatids. These are associated with wilt of oil palm (Marchitez) and coconut tree (Hartrot), and they are endemic in South America. We have studied new or doubtfully classified isolates by RAPD. We tested different primer choices and different ways to read the gels. The various dendrograms obtained have been compared. In conclusion, the influence of the crude data process on the main sub-groups of trypanosomatids is not important.

Mots-clés Agrovoc : génétique moléculaire, Phytomonas, Cocos nucifera, Elaeis guineensis, carte génétique, RAPD

Mots-clés géographiques Agrovoc : Amérique latine

Classification Agris : H20 - Maladies des plantes

Auteurs et affiliations

  • Hebrard Eugénie
  • Dollet Michel, CIRAD-CP-DDC (FRA)

Autres liens de la publication

Source : Cirad - Agritrop (https://agritrop.cirad.fr/390054/)

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