Endogenous cytokinins as biochemical markers of rubber-tree (Hevea brasiliensis) clone rejuvenation

Perrin Y., Doumas P., Lardet Ludovic, Carron Marc-Philippe. 1997. Endogenous cytokinins as biochemical markers of rubber-tree (Hevea brasiliensis) clone rejuvenation. Plant Cell, Tissue and Organ Culture, 47 : 239-245.

https://doi.org/10.1007/BF02318978

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Autre titre : Les cytokinines endogènes comme marqueurs biochimiques du rajeunissement de clones d'Hevea brasiliensis

Résumé : The endogenous levels of isopentenyladenine, isopentenyladenosine, zeatin and zeatin riboside and the ability for #in vitro# axillary shoot organogenesis and rhizogenesis were compared between mature and rejuvenated clones of #Hevea brasiliensis# (Müll. Arg.). Enhancement of the #in vitro# organogenesis ability of rubber-tree clones following somatic embryogenesis or repeated grafting onto juvenile rootstocks was accompanied by an increase of zeatin riboside levels in shoots used as starting material for #in vitro# micropropagation. Furthermore, the zeatin level, in #in vitro# shoots of clones treated by #in vitro# micrografting, and consequently capable of axillary shoot and root organogenesis, was higher than in #in vitro# shoots of non treated mature material incapable of #in vitro# organogenesis. We conclude that the endogenous zeatin-like cytokinin level (free and ribosylated forms) can be considered as a reliable marker for the recovery of #in vitro# shoot and root organogenesis after rejuvenating treatments in rubber-tree clones.

Mots-clés Agrovoc : Hevea brasiliensis, clone, cytokinine, zéatine, marqueur génétique, stade de développement, développement embryonnaire, organogénèse, culture in vitro, micropropagation, Embryogénèse somatique