Lardet Ludovic, Aguilar Maria Elena, Michaux-Ferrière Nicole, Berthouly Marc. 1998. Effect of strictly plant-related factors on the response of Hevea brasiliensis and Theobroma cacao nodal explants cultured in vitro. In Vitro Cellular and Developmental Biology. Plant, 34 : 34-40.
Autre titre : Effets de facteurs exclusivement végétaux sur la réponse d'explants nodaux cultivés in vitro chez Hevea brasiliensis et Theobroma cacao
Résumé : Nodal cuttings (primary nodal explants) were used to initiale microcutting cultures of #Hevea brasiliensis# and #Theobroma cacao#. The physiological stage of growth reached by the branches from which the explants were taken had a considerable influence on culture initiation and response. In both species, the buds of the nodal explants taken from dormant branches (stages 11, I2 and 13 in #Theobroma#; stage D in #Hevea# revealed greater opening ability #in vitro# and shoot elongation than buds taken from branches in a phase of active meristem growth in #Theobroma# (stages Fl and F2) or during a foliar growth phase in #Hevea# (stage C). In #Hevea#, the branch growth phase effect persisted into the multiplication phase when shoots developed #in vitro# were used as the source of secondary nodal explants. However, it disappeared during subcultures of primary nodal explants. In both species, the position of the nodes on the branch with respect to the apex also had an effect on culture initiation. The buds of noces furthest from the apex were more likely to develop into shoots. In #Hevea#, the position of secondary nodal explants obtained from the primary shoot was seen to vary in effect depending on growth regulator applications during primary culture. These results are analyzed in terms of variations in the balance between endogenous growth promoters/inhibitors in the buds, depending on morphogenic stages and the bud position on the branch.
Mots-clés Agrovoc : Hevea brasiliensis, Theobroma cacao, culture in vitro, explant, micropropagation, stade de développement, croissance, substance de croissance végétale
Classification Agris : F02 - Multiplication végétative des plantes
Auteurs et affiliations
- Lardet Ludovic, CIRAD-CP-HEVEA (FRA)
- Aguilar Maria Elena, CATIE (CRI)
- Michaux-Ferrière Nicole, CIRAD-AMIS-BIOTROP (FRA)
- Berthouly Marc, CIRAD-AMIS-BIOTROP (FRA)
Autres liens de la publication
- Document en bibliothèque
- Localisation du document : CD_BR6894 [(Bibliothèque de Lavalette)] ; CD_BR7315 [(Bibliothèque de Lavalette)]
Source : Cirad - Agritrop (https://agritrop.cirad.fr/390160/)
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