In vitro shoot apex micrografting of mature Acacia mangium

Monteuuis Olivier. 1996. In vitro shoot apex micrografting of mature Acacia mangium. Agroforestry Systems, 34 (2) : 213-217.

Résumé : Prospects for in vitro micrografting shoot apices of masure Acacia mangium trees were investigated with the use of 432 micrografts. Overal1 success rates of 51.5% were obtained for shoot apices ranging from 200 to 400 um in length, and a short basai wedge of underlying tissues top-grafted in aseptic conditions onto 2-to-3-month-old in vitro grown Acacia mangium seedlings. The successfully established micrografts displayed, however, substantiel variability in terms of further scion elongation as 41% of these micrografts, or 21.2% only of the total amount of the micrografts performed, had resumed growth two months after micrografting. The elongated scions exhibited different types of morphology, ranging from juvenile-like type composed leaves to the predominant mature-like phyllode morphology. Side-grafting, a more difficult procedure to perform than top-grafting, or placing the micrografts for 2 weeks in darkness after grafting, did not improve the scores. Moreover, attempts to micrograft meristems (150 200 um) resulted in 5% success only.

Mots-clés Agrovoc : Acacia mangium, greffage, vitroplant, multiplication végétative, anatomie végétale, viabilité

Mots-clés géographiques Agrovoc : Asie du Sud-Est

Classification Agris : F02 - Multiplication végétative des plantes
F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Monteuuis Olivier, CIRAD-FORET-PLANTATIONS FORESTIERES (MYS)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/390171/)

Métriques

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