Characterization of phenotypically distinct strains of Xanthomonas axonopodis pv. citri from southwest Asia

Vernière Christian, Hartung J.S., Pruvost Olivier, Civerolo E.L., Alvarez Anne M., Maestri P., Luisetti Jacques. 1998. Characterization of phenotypically distinct strains of Xanthomonas axonopodis pv. citri from southwest Asia. European Journal of Plant Pathology, 104 : 477-487.

https://doi.org/10.1023/A:1008676508688

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Autre titre : Caractérisation de souches phénotypiquement distinctes de Xanthomonas campestris pv. citri provenant d'Asie du Sud Ouest

Résumé : Strains of #Xanthomonas axonopodis# pv. #citri# were isolated from Mexican lime (#Citrits aurantifolia#) trees in several countries in southwest Asia. These strains produced typical erumpent bacterial canker lesions on Mexican lime but not on grapefruit (#C. paradisi#). Lesions on grapefruit were watersoaked and blister-like in contrast to the typical erumpent lesions seen after artificial inoculation with all described pathotypes of #X. axonopodis# pv. #citri#. This group of strains hydrolysed gelatin and casein and grew in the presence of 3% NaCl as is typical of #X. axonopodis# pv. #citri# pathotype A. RFLP analyses and DNA probe hybridization assays also gave results consistent with #X. axonopodis# pv. #citri# pathotype A. Metabolic fingerprints prepared with the Biolog(R) system showed similarities as well as differences to #X. axonopodis# pv. #citri# pathotype A. In spite of the physiological and genetic similarities to pathotype A of #X. axonopodis# pv. #citri#, these strains had no or very little affinity for polyclonal antiserum prepared against any of the reference strains of #X. axonopodis# pv. #citri# and also did not react with monoclonal antibody A1, an antibody that detects all strains of pathotype A of #X axonopodis# pv. #citri#. These strains were also insensitive to bacteriophage Cp3 like #X. axonopodis# pv. #citri# pathotype A and unlike #X. axonopodis# pv. #citri# pathotype B. We conclude that these strains, designated Xcc-A*, represent a variant of #X. axonopodis# pv. #citri# pathotype-A with pathogenicity limited to #C. aurantifolia#. The existence of extensive genotypic and phenotypic variation within pathotype A of #X. axonopodis# pv. #citri# was unexpected and further complicates the systematics of this species.

Mots-clés Agrovoc : Xanthomonas campestris citri, épidémiologie, variation génétique, anticorps monoclonal, pathotype, Xanthomonas axonopodis, chancre

Mots-clés géographiques Agrovoc : Asie du Sud-Ouest

Mots-clés complémentaires : Chancre citrique

Classification Agris : F30 - Génétique et amélioration des plantes
H20 - Maladies des plantes

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