Quantitative trait dissection analysis in Eucalyptus using RAPD markers. 1. Detection of QTL in interspecific hybrid progeny, stability of QTL expression across different ages

Verhaegen Daniel, Plomion Christophe, Gion Jean-Marc, Poitel Mireille, Costa P., Kremer Antoine. 1997. Quantitative trait dissection analysis in Eucalyptus using RAPD markers. 1. Detection of QTL in interspecific hybrid progeny, stability of QTL expression across different ages. Theoretical and Applied Genetics, 95 : 597-608.

Résumé : The objective of this study was to use random amplified polymorphic DNA (RAPD) to determine the genetic location and effects of genomic regions controlling wood density, stem growth and stem form in two species of Eucalyptus. Two hundred F, trees generated from an înterspecific cross E. urophylla x E. grandis between two elite trees were used. Genetic maps were constructed for each parent with markers segregating in the 1: 1 ratio in FS progeny. A total of 86 and 92 markers distributed among 11 linkage groups covered 1295 cM and 1312 cM for the E. urophylla and E. grandis parent, respectively. Traits were measured three times up to selection age (38 months). The magnitude of the phenotypic variation explained by the joint action of the segregating quantitative trait alleles indicated that genetic factors of large effect were involved in the control of the studied characters. Several regions controlling part of the variation for the studied traits were identified by interval mapping. Some regions of the genome exerted effects on more than one trait, providing a genetic explanation for at least some of the correlation between the traits. On the basis of an age-byage analysis, a partial stability of QTL expression was observed with 68% of the QTL being expressed at two ages and 32% being age-specific. No QTL were significant for all three ages. Taking advantage of repeated measurements on the saine material across différent ages, we investigated with a maximum statistical power, the effect of marker genotype on traits, with age and QTL x age interaction effects being removed. A two-way analysis of variance made it possible to detect significant marker-trait associations over the period studied. Most of them had already been detected in the annual analysis. This result is very encouraging for the application of marker information to the early selection of hybrid trees to be vegetatively propagated for the production of clonal varieties.

Mots-clés Agrovoc : Eucalyptus, méthode statistique, marqueur génétique

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Verhaegen Daniel, CIRAD-FORET-PLANTATIONS FORESTIERES (FRA)
• Plomion Christophe
• Gion Jean-Marc, CIRAD-FORET-PLANTATIONS FORESTIERES (FRA)
• Poitel Mireille, CIRAD-FORET-PLANTATIONS FORESTIERES (FRA)
• Costa P.
• Kremer Antoine

Source : Cirad - Agritrop (https://agritrop.cirad.fr/390899/)

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