Plant regeneration from cultured protoplasts of the cooking banana cv. Bluggoe (Musa sp., ABB group)

Megia R., Haïcour R., Tizroutine S., Bui Trang Viêt, Rossignol L., Sihachakr D., Schwendiman Jacques. 1993. Plant regeneration from cultured protoplasts of the cooking banana cv. Bluggoe (Musa sp., ABB group). Plant Cell Reports, 13 : 41-44.

Résumé : Suspensions of embryogenic cells of a triploid banana (#Musa# sp., cv. Bluggoe) were initiated from the uppermost part of meristematic buds, and used as protoplast source. After 20 weeks in culture, the suspension contained a mixture of globular structures or globules and embryogenic cell clusters, as well as single cells. Two types of protoplasts were obtained from embryogenic suspension culture : small (20-30 µm) and larger (30-50 µm) protoplasts with a dense cytoplasm and large starch grains respectively. The small protoplasts probably originated from embryogenic cell clusters, and also from pseudocambial cells of globules, while larger protoplasts were probably released from oval starchy cells and those of the globule peripheral area. In co-culture with a suitable feeder, consisting of suspensions of diploid banana cells, the protoplasts of triploid banana reformed the cell wall within 24 h and underwent sustained divisions leading to the formation of small clusters of 2-3 cells within 7 days. The latter developed directly into embryos without passing through an apparent callus phase, 10% of such embryos gave rise to plantlets when subcultured in 2.2 µM 6benzylaminopurine and 2 µM 4 amino-3,5,6-trichloropicolinic acid for I week, before transfer to MS medium containing 10 µM 6-benzylaminopurine. The rest of the embryos underwent intensive direct secondary embryogenesis which could lead to the formation of plantlets with a frequency of up to 50% upon further transfer to hormone-free medium.

Mots-clés Agrovoc : Musa, Embryogénèse somatique, protoplaste

Classification Agris : F02 - Multiplication végétative des plantes
F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Megia R.
• Haïcour R.
• Tizroutine S.
• Bui Trang Viêt
• Rossignol L.
• Sihachakr D.
• Schwendiman Jacques

Source : Cirad - Agritrop (https://agritrop.cirad.fr/391012/)

Métriques

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