Polymerase chain reaction as a diagnosis tool for detecting trypanosomes in naturally infected cattle in Burkina Faso

Solano Philippe, Michel Jean-François, Lefrançois Thierry, De La Rocque Stéphane, Sididé I., Zoungrana Adrien. 1999. Polymerase chain reaction as a diagnosis tool for detecting trypanosomes in naturally infected cattle in Burkina Faso. Veterinary Parasitology, 86 : 95-103.

https://doi.org/10.1016/S0304-4017(99)00137-5

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Résumé : African animal trypanosomoses constitute the most important vector-borne cattle diseases in sub-Saharan Africa. Generally it is considered that there is a great lack of accurate tools for the diagnosis of the disease. During a trypanosomosis survey in the agro-pastoral zone of Sideradougou, Burkina Faso, 1036 cattle were examined for trypanosomes using microscopy. The PCR was applied on a subset of 260 buffy-coat samples using primers specific for Trypanosoma congolense savannah and riverine-forest groups, T vivax, and T brucei. Parasitological examination and the molecular technique were compared, showing a better efficiency of the latter. In the near future, the PCR is likely to become an efficient tool to estimate the prevalence of African trypanosomoses in affected areas.

Mots-clés Agrovoc : Protozoa, bovin, PCR, Trypanosoma, Trypanosoma congolense, Trypanosoma vivax, Trypanosoma brucei, trypanosomose, diagnostic, vecteur de maladie

Mots-clés géographiques Agrovoc : Burkina Faso

Classification Agris : L72 - Organismes nuisibles des animaux

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