Evidence of somatic embryogenesis from root tip explants of the rattan Calamus manan

Goh Doreen Kim Soh, Michaux-Ferrière Nicole, Monteuuis Olivier, Bon Marie-Claude. 1999. Evidence of somatic embryogenesis from root tip explants of the rattan Calamus manan. In Vitro Cellular and Developmental Biology. Plant, 35 (5) : 424-427.

https://doi.org/10.1007/s11627-999-0060-7

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Résumé : Somatic embryogenesis of #Calamus manan#, a single-stemmed rattan species, in tissue culture was scientifically demonstrated for the first time. Root tips of #in vitro# plantlets produced friable callus when the explants were cultivated for several mo. on a Murashige and Skoog induction medium containing 7.5 mg Picloram per 1 (31.1 =M). Histological analyses established the presence of proembryos within the callus which differentiated subsequently into somatic embryos using the same culture medium. Histological examination revealed that these somatic embryos completely lacked starch and protein reserves, which did not prevent them, however, from germinating, and showing bipolar development. These somatic embryos further developed into young plants, similarly to zygotic embryos.

Mots-clés Agrovoc : Calamus (palmae), Embryogénèse somatique, analyse histocytologique, culture in vitro

Mots-clés complémentaires : Calamus manan

Classification Agris : F02 - Multiplication végétative des plantes

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