Direct sowing of Coffea arabica somatic embryos mass-produced in a bioreactor and regeneration of plants

Etienne Barry D., Bertrand Benoît, Vasquez Nelly, Etienne Hervé. 1999. Direct sowing of Coffea arabica somatic embryos mass-produced in a bioreactor and regeneration of plants. Plant Cell Reports, 19 (2) : 111-117.

Autre titre : Semis direct d'embryons somatiques de Coffea arabica produits en masse dans un bioréacteur et régénération des plants

Résumé : The effect of germination conditions on the morphology of #Coffea arabica# L. somatic embryos mass-produced in a 1-l temporary immersion bioreactor (RITA) was studied with emphasis on direct sowing in soil. Using germinated embryos, direct sowing resulted in a highly successful conversion of embryos into plants. A culture density above 1600 embryos per 1-1 bioreactor positively affected embryo morphology by causing higher embryonic axis elongation (+4-5 mm). At this density, the addition of a high concentration of sucrose (234 mM) 2 weeks before sowing promoted an increase in effective plant conversion in soil (78%) and a vigorous vegetative growth of the resulting plants. Furthermore, direct sowing reduced handling time to 13% and shelving area requirements to 6.3% of the values obtained by conventional acclimatization of plants developed on gel media.

Mots-clés Agrovoc : Coffea arabica, embryon somatique, bioréacteur, germination, milieu de culture, facteur du milieu, adaptation, semis direct, développement biologique

Classification Agris : F02 - Multiplication végétative des plantes
F62 - Physiologie végétale - Croissance et développement

Auteurs et affiliations

• Etienne Barry D.
• Bertrand Benoît, CIRAD-CP-CAFE (CRI) ORCID: 0000-0003-1969-3479
• Vasquez Nelly, CATIE (CRI)
• Etienne Hervé, CIRAD-CP-CAFE (CRI) ORCID: 0000-0002-6208-9982

Source : Cirad - Agritrop (https://agritrop.cirad.fr/392545/)

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