Chaïr Hâna, Kuhapituktum R., Attathom T., Attathom S..
1997. Agrobacterium mediated transformation of Sri Somrong 60, a Thai cotton variety.
In : Abstracts. Regional conference and workshop on "cotton research and development in South-East Asia" and the third national cotton conference. Kasetsart University; CIRAD-CA; Ministry of Foreign Affairs
Résumé : Techniques were developed for embryogenic callus induction, transformation and regeneration of Sri Somrong 60, a Thai cotton variety. Somatic embryogenesis was obtained by culturing hypocotyl explants onto zeatin: NAA containing MS medium and subculturing onto hormone free transferring medium. This methods yielded 23.52-50% embryogenic callus induction. Transformation of Sri Somrong 60 with GUS gene and synthetic cryIA(b) gene was accomplished by using Aggrobacterium tumefaciens strain C 58 at 1150 dilution with the addition of 100 micronM acetosyringone to the medium during inoculation and incubation periods. Fragments of crylA(b) gene were amplified from DNA isolated from transformed calli and regenerated plants suggesting that transformation of Sri Somrong 60 with CryIA(b) gene was successful. (Résume d'auteur)
Classification Agris : F02 - Multiplication végétative des plantes
F30 - Génétique et amélioration des plantes
Auteurs et affiliations
- Chaïr Hâna, CIRAD-CA-COTON (THA)
- Kuhapituktum R., Kasetsart University (THA)
- Attathom T.
- Attathom S.
Source : Cirad - Agritrop (https://agritrop.cirad.fr/392565/)
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