Gargani Daniel, Menara C., Segur C., Dollet Michel.
1992. In vitro cultivation of Phytomonas from latex and phloem-restricte d Phytomonas.
In : Phytomonas workshop = [Second atelier international Phytomonas]
Autre titre : Culture in vitro de Phytomonas à partir de latex et de phloème limité de Phytomonas
Résumé : The #in vitro# culture of #Phytomonas# was the gain obstacle to their study. In and after 1982 we were able to grow #Phytomonas# from laticiferous plants: #Euphorbia pinea#, #E. hirta#, #E. hyssopifolia#, #Mandevilla scabra#, from different areas of the world. But phloem-restricted #Phytomonas# remained difficult to grow and it was not until 1986 we obtained the first primoculture of #Phytomonas# associated with Hartrot disease of coconut, in a medium containing insect cells. After several passages axenic cultures have been obtained. Today we are growing 9 isolates of Hartrot of French Guiana, one from Brazil and two from Venezuela. #Phytomonas# associated with Marchitez disease of oil palm were cultured for the first time in 1989. Four isolates from Colombia are now being cultured. Four of these phloem-restricted isolates have been cloned (37 clones). Recently #Phytomonas# (apparently phloem-restricted) were discovered in Grenada by P. HUNT in #Alpinia purpurata#. Two isolates were obtained in insect cell cultures in the laboratory.
Mots-clés Agrovoc : tissu vasculaire, phloème, Phytomonas, culture in vitro, latex, Euphorbia, expérimentation en laboratoire
Mots-clés géographiques Agrovoc : France
Classification Agris : H20 - Maladies des plantes
Auteurs et affiliations
- Gargani Daniel
- Menara C.
- Segur C.
- Dollet Michel
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/400820/)
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