Comparative histology of microprojectile-mediated gene transfer to embryogenic calli in japonica rice (Oryza sativa L.) : influence of the structural organization of target tissues on genotype transformation ability

Bec S., Chen Li, Michaux-Ferrière Nicole, Legavre Thierry, Fauquet C., Guiderdoni Emmanuel. 1998. Comparative histology of microprojectile-mediated gene transfer to embryogenic calli in japonica rice (Oryza sativa L.) : influence of the structural organization of target tissues on genotype transformation ability. Plant Science, 138 : 177-190.

Autre titre : Histologie comparative du tranfert de gènes par microprojectiles sur des cals embryonnaires de la variété de riz japonica (Oryza sativa L.) : influence de l'organisation structurelle des tissus cibles sur la capacité de transformation du génotype

Résumé : The histology and time course of hygromycin-resistant callus formation from bombarded, seed embryo scutellum derived embryogenic nodular units (ENUs) were investigated in four japonica rice cultivars (Taipei309, IDSA6, IR47686, IRAT361) exhibiting contrasting amenabilies in a microprojectile-mediated transformation system. ENUs from the above genotypes collected at a stage considered suitable for bombardment, exhibited contrasted organization in their peripheral regions. This organization was found to influence the hygromycin concentration requested to stop ENU growth, along with the amenability of transformed cells to evolve into isolated, clonal, transgenic resistant calli, from the rest of the ENU. An average of 2.5, 4.3, and 3.2 hygromycin resistant calli were produced per responding ENU in Taipei 309, IDSA6 and IR47686, respectively. We obtained histological and molecular evidence that the hygromycin resistant calli arising from a single bombarded ENU may have been formed through independent transformation events

Mots-clés Agrovoc : Oryza sativa, culture de tissu, cal, embryon somatique, transfert de gène, biolistique, transformation génétique, analyse histocytologique

Classification Agris : F50 - Anatomie et morphologie des plantes
F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Bec S.
• Chen Li, ILTAB (USA)
• Michaux-Ferrière Nicole
• Legavre Thierry, CIRAD-AMIS-BIOTROP (FRA)
• Fauquet C.
• Guiderdoni Emmanuel, CIRAD-AMIS-BIOTROP (FRA)

Source : Cirad - Agritrop (https://agritrop.cirad.fr/401137/)

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