Urbino Cica, Gérion A., Caruana Marie-Line.
1998. Molecular and biological characterization of three isolates of geminivirus infecting tomato in Guadeloupe.
In : 2nd international workshop on Bemisia and geminiviral diseases. Program and abstracts = [Deuxième séminaire international sur les maladies à Bemisia et gemini virus. Programme et résumés]. Mayer R.T. (ed.), Maxwell D.P. (ed.)
Autre titre : Caractérisation biologique et moléculaire de trois isolats de geminivirus infectant la tomate en Guadeloupe
Résumé : Since 1989, tomato fields in Guadeloupe have been infested by a geminivirus disease. Symptoms are yellow mosaic, chlorotic mottling, leaf rolling and stunting. A survey showed that the disease was present over all the island. Cloning and sequencing of an isolate lead to the identification of a bipartite geminivirus close to potato yellow mosaic virus (PYMV-G) (Polston et al, 1998). In this study, three other virus isolates were collected from tomato in Grande-Terre and on the leeward coast of Basse-Terre and from whiteflies (Bemisia argentifolii) in Grande-Terre. They were characterized biologically (mechanical and vector transmission, host range) and molecularly. PCR primers based on conserved sequences were used to amplify and sequence the intercistronic region and the N-terminal region of the coat protein gene (CP). Sequence comparisons between these isolates and the clone of PYMV-G were established in order to confirm the occurrence of PYMV in Guadeloupe.
Mots-clés Agrovoc : Geminiviridae, identification, Solanum
Mots-clés géographiques Agrovoc : Guadeloupe, France
Classification Agris : H20 - Maladies des plantes
Auteurs et affiliations
- Urbino Cica ORCID: 0000-0002-6215-9389
- Gérion A.
- Caruana Marie-Line, CIRAD-FLHOR-BPA (GLP) ORCID: 0000-0003-4486-2449
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/401219/)
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