De Touchet B., Duval Yves, Pannetier Catherine.
1990. Oil palm (Elaeis guineensis Jacq.) regeneration from embryogenic suspension culture.
In : Abstracts VIIth international congress on plant tissue and cell culture = [Résumés du 7ème congrès international sur les cultures de tissus et de cellules des plantes]. IAPTC
Autre titre : Régénération végétale à partir de cultures de suspensions embryogéniques de palmier à huile (E. guineensis Jacq.)
Résumé : Oil palm, vegetative propagation on solid medium is performed on a large scale for several years. In order to improve efficiency of the process, investigations on liquid medium were carried out. Embryogenic calli produced from leaf explants of adult oil palm were placed in Murashige and Skoog's modified liquid medium containing 2,4-dichlorophenoxyacetic acid (2,4-D), cytokinins and activated charcoal. White globular aggregates were released in the medium during the first subculture and proliferated. After removal of the callus tissue, a suspension was established. It was made of meristematic clumps, as confirmed by histological examinations. Culture conditions were studied to improve the proliferation rate. The liquid culture has been maintained for over 18 months without any loss of embryogenic capacity. Following transfer to an hormone-free liquid medium, proliferation stopped and differentiation of clumps towards embryos has been observed. After plating on the some medium, the maturation of embryos was achieved and regenerated plants were successfully transferred to soil.
Mots-clés Agrovoc : Elaeis guineensis, multiplication végétative, Embryogénèse somatique, milieu de culture
Classification Agris : F02 - Multiplication végétative des plantes
Auteurs et affiliations
- De Touchet B.
- Duval Yves
- Pannetier Catherine
Autres liens de la publication
Source : Cirad - Agritrop (https://agritrop.cirad.fr/401257/)
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