Estimation of Frankia growth using Bradford protein and INT reduction activity estimations : application to inoculum standardization

Prin Yves, Neyra Marc, Diem Hoang Gia. 1990. Estimation of Frankia growth using Bradford protein and INT reduction activity estimations : application to inoculum standardization. FEMS Microbiology Letters, 69 (1-2) : 91-95.

Autre titre : Estimation de la croissance de Frankia utilisant la protéine Bradford et estimations de la réduction d'activité INT : application à la standardisation d'un inoculum

Résumé : The growth of Frankia spp. strain ORS 020607 in BAP medium was studied by using two methods simultaneously: determination of Bradford protein content and INT (2-(p-iodophenyl-3-(p-nitrophenyl)-5-phenyl tetrazoliumchloride) reduction activity (IRA). With the latter test, red formazan crystals formed intracellularly were extracted with methanol. Colouration intensity was estimated by absorbance spectrophotometry at 490 nm. The protein content and IRA of the culture were monitored for 96 days. IRA appeared to reflect the 'metabolically active' biomass of Frankia more accurately than the Bradford protein estimations.

Mots-clés Agrovoc : inoculation, protéine, métabolisme, méthode, Bacteria, biomasse, croissance, Actinomycetales, milieu de culture, Frankia

Mots-clés complémentaires : Inoculum, Estimation

Classification Agris : P06 - Sources d'énergie renouvelable
P34 - Biologie du sol
K10 - Production forestière
F62 - Physiologie végétale - Croissance et développement

Auteurs et affiliations

• Prin Yves ORCID: 0000-0002-3706-0045
• Neyra Marc
• Diem Hoang Gia

Source : Cirad - Agritrop (https://agritrop.cirad.fr/418795/)

Métriques

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