Plant regeneration from hybrid cell suspension derived protoplasts of Mediterranean rice (Oryza sativa L. cv. Miara)

Guiderdoni Emmanuel, Chaïr Hâna. 1992. Plant regeneration from hybrid cell suspension derived protoplasts of Mediterranean rice (Oryza sativa L. cv. Miara). Plant Cell Reports, 11 (12) : 618-622.

Autre titre : Régénération de plantes à partir de cellules d'haploïdes en suspension issues de protoplastes de riz méditerranéen (Oryza sativa L. cv. Miara)

Résumé : More than 750 plants were regenerated f rom protoplasts isolated from microspore callus-derived cell suspensions of the Mediterranean japonica rice Miara, using a nurse-feeder technique and N6-based culture medium. The mean plating efficiency and the mean regeneration ability of the protocalluses were 0.5% and 49% respectively. Flow cytometric evaluation of the DNA contents of 7 month old-cell and protoplast suspensions showed that they were still haploid. Contrastingly, the DNA contents of leaf cell nuclei of the regenerated protoclones ranged from 1C to 5C including 60% 2C plants. This was consistent with the morphological type and the fertility of the mature plants. These results and the absence of chimeric plants suggest that polyploidization occurred during the early phase of protoplast culture.

Résumé (autre langue) : La régénération de plantes viables in vitro est encore perfectible chez certaines espèces. Les auteurs ont obtenu 750 diploïdes de riz japonica (variété italienne) à partir de protoplastes issus de cellules haploïdes. L'analyse de la teneur en ADN et l'absence de chimère suggèrent que la polyploïdisation a eu lieu durant une phase précoce de la culture de protoplastes.

Mots-clés Agrovoc : Oryza sativa, protoplaste, culture de cellule, haploïdie

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Guiderdoni Emmanuel
• Chaïr Hâna

Source : Cirad - Agritrop (https://agritrop.cirad.fr/419275/)

Métriques

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