Biomolecular markers : applications, limits and perspectives in cultivars and rootstocks identification

Luro François, Ollitrault Patrick. 1997. Biomolecular markers : applications, limits and perspectives in cultivars and rootstocks identification. In : 5ème Congrès international des pépinièristes des agrumes (Montpellier, 5-7 mars 1997), résumés = 5th International congress of citrus nurserymen (montpellier, 5-7 March 1997), abstracts ; 5. Congreso mundial de viveritas de citricos. ISCN. Montpellier : CIRAD, 1 p. World Congress of the International Society of Citrus Nurserymen. 5, Montpellier, France, 5 Mars 1997/8 Mars 1997.

Autre titre : Marqueurs biomoléculaires : applications, limites et perspectives dans l'identification des cultivars et des porte-greffes

Résumé : The detection and analysis of DNA polymorphisms in crops is an essential component of marker-assisted selection and cultivar identification in plant breeding. We have explored several molecular techniques which allow to detect a DNA polymorphism: isozymes, amplification of microsatellite interspaced DNA sequences (AMIS), random PCR amplified polymorphic DNA (RAPD) and restriction fragment lenght polymorphism (RFLP). Number of problems in citrus breeding and systematics can be approached using these techniques: (i) molecular markers provide a more direct method to screen a large number of plants, at early development stages; (ii) genetic origin identification (paternity or nucellar/zygotic origin); (iii) genetic linkage map construction to locate genes involved in the development of agronomically interesting characters; markers associated with these genes could then be used for early selection of progenies and possible progenitors for breeding programmes; (iiii) in new breeding schemes employing somatic hybridization these molecular markers are essential to control the genetic composition of the vitroplantelets and calli arising from protoplast fusion. Actually, the microsatellite marker seems to be the most valuable tool in genetic studies and appears very promising in all breeding applications.

Mots-clés Agrovoc : Citrus, marqueur génétique, RAPD, RFLP, PCR, porte greffe, variété, identification

Classification Agris : F30 - Génétique et amélioration des plantes

Auteurs et affiliations

• Luro François
• Ollitrault Patrick, CIRAD-FLHOR-AAF (GLP) ORCID: 0000-0002-9456-5517

Source : Cirad - Agritrop (https://agritrop.cirad.fr/465265/)

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